Effect of 7-10 days of cycle ergometer exercise on skeletal muscle GLUT-4 protein content.
Effect of 7-10 days of cycle ergometer exercise on skeletal muscle GLUT-4 protein content.
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7-10 天的自行车测功机运动对骨骼肌 GLUT-4 蛋白含量的影响。
DOI：
10.1152/jappl.1995.79.5.1562
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Spina,RJ
中科院分区：
文献类型：
--
作者：
Gulve,EA;Spina,RJ
先前在动物和人类中的研究表明，持续数周至数月的耐力运动训练方案诱导骨骼肌GLUT-4蛋白浓度的适应性增加。一般认为，GLUT-4浓度的增加是对训练的长期适应。本研究探讨了7-10天的自行车运动是否会引起骨骼肌GLUT-4水平的增加。8名健康受试者（4名男性，4名女性），年龄31 +/- 2（SE）岁，每天在65-70%的峰值摄氧量（VO 2 peak）下运动2小时，连续7天（n = 3）或10天（n = 5）。肌肉活检（股外侧肌）前获得的运动计划开始和36-48小时后的最后一轮运动。使用GLUT-4特异性抗血清通过Western印迹法定量葡萄糖转运蛋白。训练后，VO 2峰值增加了10%（从3.0 +/- 0.2升/分增加到3.3 +/- 0.2升/分; P < 0.01）。训练后体重没有变化（训练前74.3 +/- 4.6 kg，训练后75.0 +/- 4.2 kg）。肌肉GLUT-4免疫反应性增加98%（从584 +/- 50到1，154 +/- 40计数/分钟125 I/25微克蛋白; P < 0.001）。VO 2峰值和GLUT-4蛋白的增加在7天和10天的训练中是相似的。这些结果表明，给予足够的训练刺激，骨骼肌GLUT-4蛋白的适应发生得非常迅速。此外，运动7-10天后GLUT-4的增加与采用长期训练方案的研究报告一样大。
Previous studies in animals and humans have shown that endurance exercise-training protocols of several weeks to many months in duration induce adaptive increases in skeletal muscle GLUT-4 protein concentration. It is generally assumed that the increase in GLUT-4 concentration is a long-term adaptation to training. The present study examined whether 7–10 days of cycle ergometer exercise could induce increases in skeletal muscle GLUT-4 levels. Eight healthy subjects (4 men, 4 women) aged 31 +/- 2 (SE) yr exercised 2 h daily at 65–70% of peak O2 uptake (VO2peak) for either 7 (n = 3) or 10 (n = 5) consecutive days. Muscle biopsies (vastus lateralis) were obtained before initiation of the exercise program and 36–48 h after the final bout of exercise. Glucose transporter protein was quantitated by Western blotting using antiserum specific for GLUT-4. VO2peak was increased by 10% (from 3.0 +/- 0.2 to 3.3 +/- 0.2 l/min; P < 0.01) in response to the training. Body weight did not change (74.3 +/- 4.6 before vs. 75.0 +/- 4.2 kg after) as a result of training. Muscle GLUT-4 immunoreactivity was increased 98% (from 584 +/- 50 to 1,154 +/- 40 counts per minute 125I/25 micrograms protein; P < 0.001) in response to training. Increase in VO2peak and GLUT-4 protein were similar for 7 and 10 days of training. These results suggest that, given an adequate training stimulus, adaptations in skeletal muscle GLUT-4 protein occur very rapidly. Furthermore, the increase in GLUT-4 after 7–10 days of exercise is as large as that reported in studies employing long-term training protocols.