On-site testing of saliva and sweat with Drugwipe and determination of concentrations of drugs of abuse in saliva, plasma and urine of suspected users
On-site testing of saliva and sweat with Drugwipe and determination of concentrations of drugs of abuse in saliva, plasma and urine of suspected users
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DOI：
10.1007/s004140050287
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发表时间：
2000-04-01
影响因子：
2.1
通讯作者：
van Haeren, C
中科院分区：
文献类型：
--
作者：
Samyn, N;van Haeren, C
潜在的吸毒者自愿参加了一项关于非仪器免疫测定药物擦拭（Securetec，德国）用于筛查唾液和汗液中的可卡因、阿片类药物、安非他明和大麻素的有用性的比利时研究。如果其中一项筛查（尿液、口服液、汗液）结果呈阳性，则采集血液和唾液。现场Drugwipe结果与实验室唾液Drugwipe结果、相应唾液、血浆和尿液样品GC/MS结果及取样时药理作用相关。事实证明，药物擦拭法对于检测近期可卡因（n = 6）和安非他明（n = 15）的滥用具有足够的灵敏度，无论该仪器是在舌头上擦拭还是在身体表面擦拭，或者当唾液样本应用于擦拭部分时。在5名潜在可卡因使用者中，唾液可卡因浓度超过1000纳克/毫升。安非他命组13例唾液中安非他命、MDMA或两者浓度均高（100 ~ 1000 ng/ml）。对于可卡因和安非他明，Drugwipe的阳性评分与三种体液的GC/MS结果相匹配。最近的海洛因滥用（n = 5）在一定程度上可以通过舌上的Drugwipe样本来证明，但只有唾液中MAM (> 500 ng/ml和吗啡（> 500 ng/ml）浓度最高的两名受试者呈阳性。如果考虑到比利时在阿片类药物影响下驾驶的法定临界值（血浆中游离吗啡20纳克/毫升），则只有3名受试者在法律上呈阳性。对于大麻素ill = 15)。观察到假阴性甚至假阳性。唾液可以作为一种有用的分析矩阵，用于检测最近滥用的药物。
Potential drug users participated voluntarily in a Belgian study on the usefulness of the non-instrumental immunoassay Drugwipe (Securetec, Germany) for the screening of cocaine, opiates, amphetamine and cannabinoids in saliva and sweat. Lf one of the screening assays (urine, oral fluid, sweat) showed a positive result, blood and saliva were collected. The on-site Drugwipe results were correlated with the Drugwipe results for saliva in the laboratory and with the GC/MS results of the corresponding saliva, plasma and urine samples and pharmacological effects at the time of sampling. The Drugwipe assay proved to be sufficiently sensitive for the detection of recent cocaine (n = 6) and amphetamine (n = 15) abuse, whether the device was wiped on the tongue or on the surface of the body, or when a saliva sample was applied to the wiping part. In five of the sis potential cocaine users, the saliva concentrations of cocaine exceeded 1000 ng/ml. In the amphetamine group, the saliva concentrations of amphetamine, MDMA or both were high (> 1000 ng/ml) in 13 subjects. For cocaine and amphetamine, the positive scores for Drugwipe matched the GC/MS results for the three body fluids. Recent heroin abuse (n = 5) could be demonstrated to some extent with Drugwipe on samples from the tongue but only the two subjects with the highest saliva concentrations of MAM (> 500 ng/ml and morphine (> 500 ng/ml) were positive. If the legal cut-off value for driving under the influence of opiates in Belgium (20 ng/ml of free morphine in plasma) was taken into account, only three subjects would have been legally positive. For cannabinoids ill = 15). false negatives and even some false positives were observed. Saliva can be considered as a useful analytical matrix for the detection of drugs of abuse after recent abuse when analysed with GC/MS.