Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces
Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces
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DOI：
10.1016/s0040-4039(01)00108-3
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发表时间：
2001-03-11
影响因子：
1.8
通讯作者：
Graham, D
中科院分区：
文献类型：
--
作者：
Brown, R;Smith, WE;Graham, D
已经开发了通过亚磷酰胺化学将苯并三唑部分添加到寡核苷酸的5 '-末端的方法。在苯并三唑上使用单甲氧基三苯甲基保护基团允许通过反相HPLC进行快速柱上脱三苯甲基纯化。修饰后的寡核苷酸在银胶中获得了表面增强拉曼散射（Sers）。(C)2001爱思唯尔科技有限公司版权所有。
A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid. (C) 2001 Elsevier Science Ltd. All rights reserved.