Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube.
Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube.
复制标题
DOI：
10.1242/dev.191494
复制
发表时间：
2020-12-23
期刊：
影响因子：
--
通讯作者：
Clarke JDW
中科院分区：
文献类型：
--
作者：
Symonds AC;Buckley CE;Williams CA;Clarke JDW
使用斑马鱼神经管作为模型，我们揭示了允许在最初未极化的实体器官中心生成两个相对的顶端上皮表面的体内机制。我们发现 Mpp5a 和 Rab11a 在协调同侧连接带的生成同时释放跨组织中心的对侧粘连方面发挥双重作用。我们表明，Mpp5a 和 Rab11a 介导的细胞间粘附的解决对于中线管腔开放都是必要的，并且有助于上皮组织的后期维持。我们认为 Mpp5a 和 Rab11a 的这些作用都是通过跨膜蛋白 Crumbs 发挥作用的。鉴于最近发表的一篇相互矛盾的出版物，我们还澄清 Mpp5a 的连接重塑作用并非特定于分裂细胞。摘要：使用斑马鱼神经管作为模型，我们揭示了允许在最初未极化的实体器官中心生成两个相对的顶端上皮表面的体内机制。
Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that Mpp5a and Rab11a play a dual role in coordinating the generation of ipsilateral junctional belts whilst simultaneously releasing contralateral adhesions across the centre of the tissue. We show that Mpp5a- and Rab11a-mediated resolution of cell-cell adhesions are both necessary for midline lumen opening and contribute to later maintenance of epithelial organisation. We propose that these roles for both Mpp5a and Rab11a operate through the transmembrane protein Crumbs. In light of a recent conflicting publication, we also clarify that the junction-remodelling role of Mpp5a is not specific to dividing cells. Summary: Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms that allow the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised solid organ.