Low Intensity Vibrations Augment Mesenchymal Stem Cell Proliferation and Differentiation Capacity during in vitro Expansion
Low Intensity Vibrations Augment Mesenchymal Stem Cell Proliferation and Differentiation Capacity during in vitro Expansion
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DOI：
10.1038/s41598-020-66055-0
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发表时间：
2020-06-10
影响因子：
4.6
通讯作者：
Uzer, Gunes
中科院分区：
文献类型：
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作者：
Bas, Guniz;Loisate, Stacie;Uzer, Gunes
作为运动的主要组成部分，机械信号，当以低强度振动（LIV）的形式应用时，可以增加间充质干细胞（MSC）的成骨和增殖。虽然人们普遍认为运动可以有效地对抗肌肉骨骼系统衰老的有害影响，但长期运动如何影响干细胞衰老（以增殖和分化能力降低为典型特征）还没有得到很好的探讨。作为了解长期应用机械信号对干细胞功能影响的第一步，我们研究了LIV在MSCs体外扩增过程中的作用。在体外细胞扩增条件下，原代MSCs接受对照或每天两次的LIV方案，最多可进行60次细胞传代（P60）。在早期传代（EP）和晚期传代（LP）时评估LIV效应。在实验结束时，暴露于LIV的P60培养物保持了28%的细胞加倍增加和39%的衰老相关的β -半乳糖苷酶活性降低(p
A primary component of exercise, mechanical signals, when applied in the form of low intensity vibration (LIV), increases mesenchymal stem cell (MSC) osteogenesis and proliferation. While it is generally accepted that exercise effectively combats the deleterious effects of aging in the musculoskeletal system, how long-term exercise affects stem cell aging, which is typified by reduced proliferative and differentiative capacity, is not well explored. As a first step in understanding the effect of long-term application of mechanical signals on stem cell function, we investigated the effect of LIV during in vitro expansion of MSCs. Primary MSCs were subjected to either a control or to a twice-daily LIV regimen for up to sixty cell passages (P60) under in vitro cell expansion conditions. LIV effects were assessed at both early passage (EP) and late passage (LP). At the end of the experiment, P60 cultures exposed to LIV maintained a 28% increase of cell doubling and a 39% reduction in senescence-associated beta -galactosidase activity (p