Analytical, Nutritional and Clinical Methods Section Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures
Analytical, Nutritional and Clinical Methods Section Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures
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发表时间：
1999
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通讯作者：
R. V. D. Berg;H. Berg;A. Bast
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作者：
R. V. D. Berg;H. Berg;A. Bast
TEAC（Trolox当量抗氧化能力）测定是基于2，2，0 -连氮基双-（3-乙基苯并噻唑啉-6-磺酸盐）自由基阴离子（ABTS）的清除。在这份报告中，我们描述了一个修改的基础上预生成的ABTS自由基阴离子与热不稳定的偶氮化合物，2，2-O-偶氮双-（2-脒基丙烷）盐酸（ABAP）。这种修改使得测定不太容易受到人为因素的影响，例如对自由基生成过程的影响。对于测试的大多数抗氧化剂，观察到两相反应模式，即快速和缓慢的清除速率。我们评估了应用程序的测定与亲脂性和亲水性化合物的抗氧化能力。几种有机溶剂，与水相容，与α-生育酚，槲皮素和β-胡萝卜素进行了测试。结果表明，TEAC在不同的溶剂中均发生变性。在标准化条件下，可以证明从单个抗氧化剂获得的TEAC的加和性。这可能使应用该测定法鉴定“未知"抗氧化剂成为可能。#1999爱思唯尔科技有限公司版权所有。
The TEAC (Trolox equivalent antioxidant capacity) assay is based on scavenging of 2,2 0 -azinobis-(3- ethylbenzothiazoline-6sulfonate) radical anions (ABTS ˇ ). In this report we describe a modification based on pre-generation of the ABTS radical anions with a thermolabile azo compound, 2,2 0 -azobis- (2-amidinopropane)HCl (ABAP). This modification makes the assay less susceptible to artefacts, e.g. influence on the radical generation process. For most antioxidants tested, a biphasic reaction pattern was seen, i.e. a fast and slow scavenging rate. We evaluated application of the assay with both lipophilic and hydrophilic compounds with antioxidant capacity. Several organic solvents, compatible with water, were tested with a-tocopherol, quercetin and b-carotene. It was found that the TEACs diAered in various solvents. Under standardized conditions additivity of TEACs obtained from individual antioxidants could be demonstrated. This might enable application of the assay for the identification of ‘‘unknown’’ antioxidants. # 1999 Elsevier Science Ltd. All rights reserved.