Distribution of beta-tectorin mRNA in the early posthatch and developing avian inner ear
Distribution of beta-tectorin mRNA in the early posthatch and developing avian inner ear
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DOI：
10.1016/0378-5955(96)00045-7
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发表时间：
1996-07-01
期刊：
影响因子：
2.8
通讯作者：
Richardson, GP
中科院分区：
文献类型：
--
作者：
Goodyear, R;Killick, R;Richardson, GP
用原位杂交技术研究了β-tectorinmRNA在鸡胚和孵化后早期（PH）内耳中的表达。在PH鸡中，β-tectorin mRNA表达于基底乳头、位于乳头两侧的透明细胞和立方细胞、黄斑的纹状区以及位于前壶腹和后壶腹嵴中线附近的两个小细胞群。在侧壶腹中未观察到β-tectorin的表达。在PH鸡的感觉上皮中检测到β-tectorin mRNA，表达仅限于支持细胞群。在耳蜗管的发育过程中，β-tectorin的表达开始于胚胎（E）第5天和第6天之间。在E6，在整个导管长度上观察到表达，但在远端最高。到E7时，表达模式逆转，并且在耳蜗的近端最高，表明在E6和E7期间，高β-tectorin表达的波沿着乳头向远侧-近侧沿着。在耳蜗管发育过程中的任何阶段，在同源细胞中均未检测到β-tectorin mRNA的表达，表明这些细胞不合成鸟类盖膜的两种主要蛋白质之一。将不同上皮细胞中表达β-tectorin mRNA的支持细胞的分布与具有B型毛束的感觉细胞的分布进行比较，所述感觉细胞的轴连接仅限于其静纤毛的基底区域，而具有A型毛束的感觉细胞的轴连接遍布其静纤毛的整个表面。从未发现具有A型毛束的毛细胞与表达β-tectorin的支持细胞相关。虽然有一个对应的基底乳头和黄斑的椭圆囊和lagena之间的分布的支持细胞表达β-tectorin mRNA和毛细胞与B型束，这种相关性不概括到其他感觉上皮。
Expression of beta-tectorin mRNA in the inner ear of the embryonic and early posthatch (PH) chick was studied by in situ hybridisation. In the PH chick, beta-tectorin mRNA is expressed in the basilar papilla, in the clear and the cuboidal cells that lie either side of the papilla, in the striolar regions of the maculae, and in two small groups of cells lying adjacent to the midline in the cristae of the anterior and posterior ampullae. Expression of beta-tectorin is not observed in the lateral ampulla. In the sensory epithelia of the PH chick in which beta-tectorin mRNA is detected, expression is restricted to the supporting cell population. During development of the cochlear duct, beta-tectorin expression begins between embryonic (E) days 5 and 6. At E6, expression is observed throughout the length of the duct but is highest at the distal end. By E7, the pattern of expression is reversed and is highest at the proximal end of the cochlea, suggesting that a wave of high beta-tectorin expression passes disto-proximally along the papilla during E6 and E7. Expression of beta-tectorin mRNA is not detected in the homogene cells at any stage during the development of the cochlear duct, indicating that these cells do not synthesise one of the two major proteins of the avian tectorial membrane. The distribution of supporting cells expressing beta-tectorin mRNA in the different epithelia was compared with the distribution of sensory cells that have type B hair bundles, those with shaft links restricted to basal regions of their stereocilia, and sensory cells that have type A bundles, those with shaft links all over the entire surface of their stereocilia. Hair cells with type A hair bundles are never found in association with supporting cells expressing beta-tectorin. Although there is a correspondence in the basilar papilla and the maculae of the utriculus and lagena between the distribution of supporting cells expressing beta-tectorin mRNA and hair cells with type B bundles, this correlation does not generalise to the other sensory epithelia.