Cloning and tissue distribution of novel splice variants of the rat GABAB receptor
Cloning and tissue distribution of novel splice variants of the rat GABAB receptor
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DOI：
10.1006/bbrc.1998.9706
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发表时间：
1998-12-09
影响因子：
3.1
通讯作者：
Taniyama, K
中科院分区：
文献类型：
--
作者：
Isomoto, S;Kaibara, M;Taniyama, K
在筛选大鼠小脑cDNA文库时，我们发现了代谢性γ-氨基丁酸受体(GABA(B)R1)的两个新的剪接变异体，分别命名为GABA(B)R1c和GABA(B)R1d。GABA(B)R1c具有与GABA(B)R1b(GABA(B)R1b)相同的氨基酸序列，GABA(B)R1b是GABA(B)R1亚型的成员，另外还有一个93bp的插入，在GABA的第五个跨膜区RLb产生额外的31个氨基酸序列。因此，GABA、RLC在第二细胞外环和第五跨膜区可能存在结构变异。GABA(B)R1d也有一个与GABA、RLb相同的氨基酸序列和一个额外的566bp的插入，在羧基末端产生一个发散的氨基酸序列。逆转录聚合酶链式反应分析表明，GABA(B)R1c在大鼠各组织中普遍表达，在前脑、小脑、眼、肾、膀胱等组织中均有表达。GABA(B)R1亚型不仅在中枢神经系统发挥作用，而且在各种外周组织中也发挥作用。(C)1998年学术出版社。
We have identified two novel splice variants of the metabotropic gamma-aminobutyric acid receptor (GABA(B)R1), designated GABA(B)R1c and GABA(B)R1d, when screening a rat cerebellum cDNA library. GABA(B)R1c has an amino acid sequence identical to GABA(B)R1b, a member of GABA(B)R1 isoforms, and an additional 93-bp insertion that generates an additional 31-amino-acid sequence in the fifth transmembrane region of GABA,Rlb. Thus, GABA,Rlc may have a structural variation in the second extracellular loop and fifth transmembrane region. GABA(B)R1d also has an amino acid sequence identical to GABA,Rlb and an additional insertion of 566 bp that generates a divergent amino acid sequence in the carboxyl-terminal end. Reverse-transcription polymerase chain reaction analysis showed that in various rat tissues GABA(B)R1c mRNA was ubiquitously expressed and GABA(B)R1d mRNA in forebrain, cerebellum, eye, kidney, and urinary bladder. GABA(B)R1 isoforms may function not only in the central nervous system but also in various peripheral tissues. (C) 1998 Academic Press.