Comparison of two toxins produced by Clostridium difficile
Comparison of two toxins produced by Clostridium difficile
复制标题
艰难梭菌产生的两种毒素的比较
作者：
N. Taylor;G. Thorne;J. Bartlett
已证明艰难梭菌可产生一种毒素，该毒素可与先前描述的同一生物体的细胞毒素进行生化分离。这两种蛋白质在生物活性和物理性质上不同。针对第二种毒素制备的抗血清不能中和细胞毒素的生物活性，并且不能证明免疫交叉反应性。然而，这两种毒素之间可能存在某种关系，因为新描述的毒素在聚丙烯酰胺电泳上降解为两种分子，其中一种似乎与纯化的细胞毒素带一起迁移。我们建议，这种新描述的毒素被指定为毒素A，直到其主要的生物活性和物理关系的细胞毒素是确定的。该毒素在肠道疾病的生物学检测中具有活性，可能在C.艰难梭菌引起的结肠炎
Clostridium difficile was shown to produce a toxin which could be biochemically separated from the previously described cytotoxin of the same organism. The two proteins differ in biological activity and physical properties. Antiserum prepared to the second toxin does not neutralize the biological activity of the cytotoxin, and immunological cross-reactivity could not be demonstrated. However, some relationship may exist between the two toxins, since the newly described toxin degrades on polyacrylamide electrophoresis into two molecules, one of which appears to migrate with the band of purified cytotoxin. We suggest that this newly described toxin be designated toxin A until its primary biological activity and physical relationship to cytotoxin is determined. This toxin is active in biological assays of enteric disease and may play an important role in C. difficile-induced colitis.