Integrating eggshell-derived CaCO3/MgO nanocomposites and chitosan into a biomimetic scaffold for bone regeneration
Integrating eggshell-derived CaCO3/MgO nanocomposites and chitosan into a biomimetic scaffold for bone regeneration
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DOI：
10.1016/j.cej.2020.125098
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发表时间：
2020-09-01
影响因子：
15.1
通讯作者：
Fan, Xian-qun
中科院分区：
文献类型：
--
作者：
Huang, Ya-zhuo;Ji, Yong-rong;Fan, Xian-qun
创伤、肿瘤切除和先天性疾病引起的骨缺损可导致畸形和功能障碍。开发具有可调成骨和降解速率的可生物降解支架对于改善骨缺损再生是非常必要的。蛋壳是一种主要无机成分为碳酸钙的天然生物材料。在此，我们合成了MgO纳米颗粒包覆的蛋壳颗粒（定义为CaCO 3/MgO纳米复合物），并随后通过化学交联的CaCO 3/MgO纳米复合物，羧甲基壳聚糖（CMC）和骨形态发生蛋白2（BMP 2）制备仿生活性支架。结果表明，新型复合CaCO 3/MgO/CMC/BMP 2支架的模量和抗压强度均高于CMC支架。有趣的是，该复合支架显示出显著的矿化能力和强的成骨分化潜力。此外，成骨分化机制的检查清楚地表明，从复合支架中释放的Mg 2+离子和BMP 2可以激活ERK 1/2和Akt通路的磷酸化，并通过多个通路的串扰促进成骨。最后，原位大鼠颅骨缺损修复实验表明，该复合支架具有良好的修复效果。这项研究非常有前途，因为它为开发用于组织工程和再生医学的天然CaCO 3基无机纳米材料提供了新的见解。
The bone defects caused by trauma, tumor resections and congenital diseases can lead to deformity and dysfunction. Developing a biodegradable scaffold with tunable osteogenesis and degradation rates is highly essential for improving bone defect regeneration. Eggshell is a kind of natural biomaterial of which the major inorganic component is CaCO3. Herein, we synthesized MgO nanoparticle-coated eggshell particles (defined as CaCO3/MgO nanocomposites) and subsequently fabricated a biomimetic active scaffold through the chemical crosslinking of the CaCO3/MgO nanocomposite, carboxymethyl chitosan (CMC) and bone morphogenetic protein 2 (BMP2). The results showed that the modulus and compressive strength of the new composite CaCO3/MgO/CMC/BMP2 scaffold were higher than those of the CMC scaffold. Interestingly, the composite scaffold exhibited significant mineralization ability and strong osteogenic differentiation potential. Moreover, an examination of the osteogenic differentiation mechanism clearly illustrated that Mg2+ ions and BMP2 released from the composite scaffold could activate the phosphorylation of the ERK1/2 and Akt pathways and promote osteogenesis through the crosstalk of multiple pathways. Finally, an in-situ rat calvarial defect repair experiment showed excellent repair results of the composite scaffold. This study is very promising, as it provides new insights for developing natural CaCO3-based inorganic nanomaterials for tissue engineering and regenerative medicine.