Crosstalk between bacterial conjugation and motility is mediated by plasmid-borne regulators
Crosstalk between bacterial conjugation and motility is mediated by plasmid-borne regulators
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DOI：
10.1111/1758-2229.12784
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发表时间：
2019-07-29
影响因子：
3.3
通讯作者：
Madrid, Cristina
中科院分区：
文献类型：
--
作者：
Luque, Ainara;Paytubi, Sonia;Madrid, Cristina
质粒接合是一种主要的水平基因转移机制。质粒的获得除了为受体细胞提供有利的特性（例如获得抗生素抗性）之外，还可引起细胞功能的扰动。使用IncHI 1质粒R27研究了质粒和染色体功能之间的相互作用。从几种革兰氏阴性病原体中分离的不相容性组HI 1的质粒与多药耐药性的传播有关。它们的结合受到温度的严格调节，在宿主内的温度（37摄氏度）下被抑制。在这份报告中，我们描述了在允许的温度下，当质粒R27的接合被提示时，观察到细胞运动性的降低。这种减少是介导的质粒编码的监管机构TrhR/TrhY，它与HtdA一起形成一个质粒携带的监管电路控制R27共轭。诱导R27结合所需的TrhR/TrhY负责鞭毛合成的下调和随之而来的运动性降低。TrhR/TrhY直接或间接抑制特定鞭毛σ亚基FliA的表达，并因此抑制位于鞭毛表达级联中的所有基因的表达。
Plasmid conjugation is a major horizontal gene transfer mechanism. The acquisition of a plasmid may cause a perturbation of the cell functions in addition to provide advantageous properties for the recipient cell, such as the gaining of antibiotic resistances. The interplay between plasmid and chromosomal functions has been studied using the IncHI1 plasmid R27. Plasmids of the incompatibility group HI1, isolated from several Gram-negative pathogens, are associated with the spread of multidrug resistance. Their conjugation is tightly regulated by temperature, being repressed at temperatures within the host (37 degrees C). In this report, we described that at permissive temperature, when conjugation of plasmid R27 is prompted, a reduction in the motility of the cells is observed. This reduction is mediated by the plasmid-encoded regulators TrhR/TrhY, which together with HtdA form a plasmid-borne regulatory circuit controlling R27 conjugation. TrhR/TrhY, required to induce R27 conjugation, is responsible for the downregulation of the flagella synthesis and the consequent decrease in motility. TrhR/TrhY repress, direct or indirectly, the expression of the specific flagellar sigma subunit FliA and, consequently, the expression of all genes located bellow in the flagellar expression cascade.