Molecular identification of histone acetyltransferases and deacetylases in lower plant Marchantia polymorpha
Molecular identification of histone acetyltransferases and deacetylases in lower plant Marchantia polymorpha
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DOI：
10.1016/j.plaphy.2018.10.012
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发表时间：
2018-11-01
影响因子：
6.5
通讯作者：
Chen, Zhong
中科院分区：
文献类型：
--
作者：
Chu, Jiashu;Chen, Zhong
组蛋白是核小体的核心组成部分，组蛋白尾部氨基酸残基的修饰是核小体表观遗传调控的重要机制之一。组蛋白乙酰化或去乙酰化由两组蛋白质进行：组蛋白乙酰转移酶（HAT）或组蛋白去乙酰化酶（HDAC），并且已被证明与动物和维管植物中的基因表达调控紧密相关。HAT和HDAC在非开花植物中的生物学功能在很大程度上仍然未知。我们发现地钱基因组中存在7个MpHAT基因和12个MpHDAC基因，并对HAT和HDAC家族进行了全面的蛋白质序列分析，以研究它们的潜在功能。在功能结构域分析的基础上，8个MpHAT和12个MpHDAC包含保守的功能结构域作为每个家族的定义特征。构建了MpHATs和MpHDAC沿着的系统发育树，并与来自不同植物和绿色藻类的同源物进行了比较，以阐明HAT和HDAC蛋白的进化关系。我们发现SIR 2家族和RPD 3/HDA 1超家族都具有较低的植物特异性蛋白，这表明HATs和HDACs在地钱等低等植物或藻类中具有潜在的未知功能。亚细胞定位预测表明，MpHATs和MpHDACs可能在各种细胞器中发挥作用。表达分析表明，所有MpHAT和MpHDAC基因在所有组织中表达，但在转录水平上存在差异。此外，它们的表达模式改变，以响应各种植物激素和环境胁迫处理。我们的结论是，所有的MpHATs和MpHDACs是地钱中的功能蛋白，并参与各种信号通路。地钱可能已经发展了一个复杂的组蛋白乙酰化表观遗传机制，以调节生长和发育，以及对环境的反应。
Histone is the core component of nucleosome and modification of amino acid residues on histone tails is one of the most pivotal epigenetic regulatory mechanisms. Histone acetylation or deacetylation is carried out by two groups of proteins: histone acetyltransferases (HATs) or histone deacetylases (HDACs), and has been proven to be tightly linked to regulation of gene expression in animals and vascular plants. The biological functions of HATs and HDACs in non-flowering plants remain largely unknown. We found that there are seven MpHAT genes and twelve MpHDAC genes present in the Marchantia genome, and the comprehensive protein sequence analysis of the HAT and HDAC families was introduced to investigate their potential functions. On the basis of the functional domain analysis, eight MpHATs and twelve MpHDACs contain the conserved functional domains as the defining feature of each family. Phylogenetic trees of all families of MpHATs and MpHDACs along with their homologs from different plant and green algae species were constructed to illustrate evolutionary relationship of HAT and HDAC proteins. We found both SIR2 family and RPD3/HDA1 superfamily possess lower plant-specific proteins indicating the potential unknown functions of HATs and HDACs in Marchantia and other lower plant or algae species. Subcellular localization prediction suggests that MpHATs and MpHDACs are likely functioning in various organelles. Expression analysis shows that all MpHAT and MpHDAC genes are expressed in all tissues with differences at the transcriptional level. In addition, their expression patterns were altered in response to various treatments with plant hormones and environmental stress. We concluded that all MpHATs and MpHDACs are functional proteins in Marchantia and involved in various signaling pathways. Marchantia could have developed a complex histone acetylation epigenetic mechanism to regulate growth and development, as well as responses to environment.