Effects of different quinoid redox mediators on the anaerobic reduction of azo dyes by bacteria.
Effects of different quinoid redox mediators on the anaerobic reduction of azo dyes by bacteria.
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DOI：
10.1021/es010227
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发表时间：
2002-03
影响因子：
11.4
通讯作者：
J. Rau;H. Knackmuss;A. Stolz
中科院分区：
文献类型：
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作者：
J. Rau;H. Knackmuss;A. Stolz
醌类氧化还原介质的添加到厌氧培养的各种分类学上不同的细菌物种的减少率显着增加的偶氮染料阿马兰斯。从不同的醌测试，一般蒽醌-2-磺酸盐（AQS）和指甲花醌（2-羟基-1，4-萘醌）引起的偶氮还原酶活性的增加最高。用鞘氨醇单胞菌BN 6和大肠杆菌K12对AQS和指甲花醌的作用进行了详细的研究。两种菌株在厌氧条件下还原醌类，相对活性差异显著。AQS，指甲花醌，和不同的其他醌的化学还原形式进行了测定，他们的脱色阿马兰斯红的能力，并观察到的醌的氧化还原电位和偶氮染料的还原速率之间的良好的相关性。AQS或指甲花醌的加入也提高了未驯化污泥还原偶氮染料的能力。化学纯的指甲花可以用含有大量指甲花的叶子粉末代替。
The addition of quinoid redox mediators to anaerobically incubated cultures of various taxonomically different bacterial species resulted in significantly increased reduction rates for the azo dye amaranth. From different quinones tested, generally anthraquinone-2-sulfonate (AQS) and lawsone (2-hydroxy-1,4-naphthoquinone) caused the highest increase in the azoreductase activities. The effects of AQS and lawsone were studied in greater detail with Sphingomonas xenophaga BN6 and Escherichia coli K12. Both strains reduced the quinones under anaerobic conditions with significantly different relative activities. The chemically reduced forms of AQS, lawsone, and different other quinones were assayed for their ability to decolorize amaranth, and a good correlation between the redox potentials of the quinones and the reduction rates of the azo dyes was observed. The addition of AQS or lawsone also increased the ability of unacclimated sewage sludge to reduce azo dyes. Chemically pure lawsone could be replaced by the powdered leaves of the henna plant which contain significant amounts of lawsone.