Mild electrical stimulation with heat stimulation increase heat shock protein 70 in articular chondrocyte
Mild electrical stimulation with heat stimulation increase heat shock protein 70 in articular chondrocyte
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DOI：
10.1002/jor.22307
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发表时间：
2013-06-01
影响因子：
2.8
通讯作者：
Kubo, Toshikazu
中科院分区：
文献类型：
--
作者：
Hiraoka, Nobuyuki;Arai, Yuji;Kubo, Toshikazu
本研究的目的是研究轻度电刺激 (MES) 和热应激 (HS) 对软骨细胞和关节软骨中的热休克蛋白 70 (HSP70) 的影响，该蛋白可保护软骨细胞并增强软骨基质代谢。用 MES 和/或 HS 处理兔关节软骨细胞。通过LDH测定和形态学评估安全性。通过Western blotting和实时PCR检测HSP70蛋白、泛素化蛋白和HSP70 mRNA。用 MES 和/或 HS 治疗大鼠膝关节。研究了关节软骨中的 HSP70 蛋白、泛素化蛋白、HSP70 mRNA 和蛋白聚糖核心蛋白 (PG) mRNA。在体外，HS增加HSP70 mRNA和HSP70蛋白。 MES 增强了泛素化蛋白和 HSP70 蛋白，但不增强 HSP70 mRNA。 MES+HS 上调 HSP70 mRNA 和泛素化蛋白，并显着增加 HSP70 蛋白。在体内，HS 和 MES+HS 治疗增强了 HSP70 mRNA。 HS 适度增强了 HSP70 蛋白。 MES+HS显着增加HSP70蛋白和泛素化蛋白。 MES+HS显着提高PG mRNA。本研究表明，MES与HS联合使用，可以增加软骨细胞和关节软骨中的HSP70蛋白，促进关节软骨中的软骨基质代谢。 MES 与 HS 相结合，通过在关节软骨中诱导 HSP70，可以成为治疗骨关节炎的新型物理疗法。 (c) 2013 年骨科研究学会。由 Wiley periodicals, Inc. 出版。J Orthop Res 31: 894900, 2013
The objective of this study is to investigate the effects of mild electrical stimulation (MES) and heat stress (HS) on heat shock protein 70 (HSP70), that protects chondrocytes and enhances cartilage matrix metabolism, in chondrocyte and articular cartilage. Rabbit articular chondrocytes were treated with MES and/or HS. The safeness was assessed by LDH assay and morphology. HSP70 protein, ubiquitinated proteins and HSP70 mRNA were examined by Western blotting and real-time PCR. Rat knee joints were treated with MES and/or HS. HSP70 protein, ubiquitinated proteins, HSP70 mRNA and proteoglycan core protein (PG) mRNA in articular cartilage were investigated. In vitro, HS increased HSP70 mRNA and HSP70 protein. MES augmented ubiquitinated protein and HSP70 protein, but not HSP70 mRNA. MES+HS raised HSP70 mRNA and ubiquitinated protein, and significantly increased HSP70 protein. In vivo, HS and MES+HS treatment augmented HSP70 mRNA. HS modestly augmented HSP70 protein. MES+HS significantly increased HSP70 protein and ubiquitinated proteins. PG mRNA was markedly raised by MES+HS. This study demonstrated that MES, in combination with HS, increases HSP70 protein in chondrocytes and articular cartilage, and promotes cartilage matrix metabolism in articular cartilage. MES in combination with HS can be a novel physical therapy for osteoarthritis by inducing HSP70 in articular cartilage. (c) 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 894900, 2013