The human gastrin/cholecystokinin type B receptor gene: alternative splice donor site in exon 4 generates two variant mRNAs.
The human gastrin/cholecystokinin type B receptor gene: alternative splice donor site in exon 4 generates two variant mRNAs.
复制标题
人胃泌素/胆囊收缩素 B 型受体基因：外显子 4 中的选择性剪接供体位点产生两个变异 mRNA。
DOI：
10.1073/pnas.90.19.9085
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发表时间：
1993
影响因子：
11.1
通讯作者：
Yamada,T
中科院分区：
文献类型：
--
作者：
Song,I;Brown,DR;Wiltshire,RN;Gantz,I;Trent,JM;Yamada,T
胃泌素及其羧基末端同源物胆囊收缩素（CCK）在脑和胃肠道中发挥多种生物学作用，这些生物学作用部分地通过一种或多种G蛋白偶联受体介导，所述G蛋白偶联受体对两种肽表现出相似的亲和力。通过用基于犬胃泌素受体的核苷酸序列的部分长度DNA片段筛选人EMBL噬菌体文库来分离编码人胃泌素/CCKB受体的基因组克隆。该基因包含一个1356 bp的开放阅读框架，由5个外显子和4个内含子组成，并被分配到人类染色体11 p15.4。外显子4的一个区域，编码推定的第三胞内环的一部分，似乎被选择性剪接产生两种不同的mRNA，一种含有（452个氨基酸;长同种型）和另一种缺乏（447个氨基酸;短同种型）五肽序列Gly-Gly-Ala-Gly-Pro。这两种受体亚型可能导致胃泌素和CCK介导的信号转导的功能差异。
Gastrin and its carboxyl-terminal homolog cholecystokinin (CCK) exert a variety of biological actions in the brain and gastrointestinal tract that are mediated in part through one or more G protein-coupled receptors which exhibit similar affinity for both peptides. Genomic clones encoding a human gastrin/CCKB receptor were isolated by screening a human EMBL phage library with a partial-length DNA fragment which was based on the nucleotide sequence of the canine gastrin receptor. The gene contained a 1356-bp open reading frame consisting of five exons interrupted by 4 introns and was assigned to human chromosome 11p15.4. A region of exon 4, which encodes a portion of the putative third intracellular loop, appears to be alternatively spliced to yield two different mRNAs, one containing (452 amino acids; long isoform) and the other lacking (447 amino acids; short isoform) the pentapeptide sequence Gly-Gly-Ala-Gly-Pro. The two receptor isoforms may contribute to functional differences in gastrin- and CCK-mediated signal transduction.