Gold-Immobilized Photonic Crystal Fiber-Based SPR Biosensor for Detection of Malaria Disease in Human Body
Gold-Immobilized Photonic Crystal Fiber-Based SPR Biosensor for Detection of Malaria Disease in Human Body
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DOI：
10.1109/jsen.2021.3085829
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发表时间：
2021-08-15
影响因子：
4.3
通讯作者：
Kumar, Santosh
中科院分区：
文献类型：
--
作者：
Chaudhary, Vijay Shanker;Kumar, Dharmendra;Kumar, Santosh
本文介绍了一种基于光子晶体光纤（PCF）的表面等离子体共振（SPR）生物传感器，通过测量红细胞（红细胞）的变化来早期检测人类疟疾疾病。在PCF中，两层空气孔以六角形晶格结构排列，并在PCF上使用一层金涂层薄膜来发生SPR现象。在相位匹配条件下，当表面等离子激元（SPP）模式与核心模式耦合时，就会发生这种现象。疟疾感染的红细胞样品被填充到PCF中，PCF具有自己的折射率（RI），在限制损耗测量期间移动SPR共振波长。由于感染疟疾的红细胞与正常红细胞的RI不同，感染疟疾的红细胞与正常红细胞的共振波长不同。本文的工作有助于通过测量共振波长的位移来检测疟疾感染红细胞的不同阶段，如环期、滋养体期和分裂体期。该传感器对环相、滋养体相和分裂体相红细胞在x偏振方向的波长灵敏度分别为13714.29 nm/RIU、9789.47 nm/RIU和8068.97 nm/RIU，在y偏振方向的波长灵敏度分别为14285.71 nm/RIU、10000 nm/RIU和8206.9 nm/RIU，最大检出限为0.029。基于pcf的SPR生物传感器具有较好的检测性能（检测限低、灵敏度高），适用于疟疾疾病的早期诊断。
This article presents the photonic crystal fiber (PCF) based surface plasmon resonance (SPR) biosensor for early detection of malaria disease in humans by measurement of the variation of red blood cells (RBCs). In the proposed PCF, two layers of air holes are arranged in a hexagonal lattice structure and a thin film of gold-coating is used over PCF for the occurrence of SPR phenomena. It occurs when surface plasmon polariton (SPP)-mode coupled with the core-mode during phase-matching conditions. Malaria infected RBCs samples are filled into the PCF, which have own refractive index (RI) that shift the SPR resonance wavelength during confinement loss measurement. The resonance wavelength of malaria-infected RBCs samples is different from their normal RBCs samples due to the difference in RI of infected and normal RBCs samples. The proposed work is helpful in the detection of different stages of malaria-infected RBCs such as ring phase, trophozoite phase and Schizont phase by measuring the shift in resonance wavelength. The calculated wavelength sensitivities of the proposed sensor for the ring phase, trophozoite phase and Schizont phase RBCs are 13714.29 nm/RIU, 9789.47 nm/RIU, and 8068.97 nm/RIU, respectively in x-polarized direction and 14285.71 nm/RIU, 10000 nm/RIU, and 8206.9 nm/RIU, respectively in y-polarized direction with the maximum detection limit of 0.029. The proposed PCF-based SPR biosensor is suitable for the early diagnosis of malaria disease due to its enhanced sensing performance (low detection limit and high sensitivity).