Eppikajutsuto (a Japanese herbal medicine) regulates mTOR and induces apoptosis in oral cancer cells
Eppikajutsuto (a Japanese herbal medicine) regulates mTOR and induces apoptosis in oral cancer cells
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DOI：
10.1002/tkm2.1244
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发表时间：
2020-08-01
期刊：
影响因子：
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通讯作者：
Ogawa-Ochiai, Keiko
中科院分区：
文献类型：
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作者：
Makita, Haruna;Endo, Kazuhira;Ogawa-Ochiai, Keiko
目的：日本传统（汉方）医学的有效性正逐渐引起全世界的关注。它通常被认为是有效对抗癌症的支持疗法，但汉方的抗肿瘤作用尚未完全确定。方法：以口腔鳞癌细胞系OSC-19为模型，采用细胞活力测定、蛋白质印迹法和凋亡细胞测定等方法，对eppikajutto的抗肿瘤作用进行了评价。结果：eppikajutto在体外可抑制细胞增殖，诱导细胞凋亡，并抑制mTOR的激活。结果表明，eppikajutsu可能通过抑制mTOR活化而对OSC-19表现出体外抗肿瘤作用。然而，需要进行额外的体外和体内研究来证实eppikajututto的抗肿瘤作用。
Aim: The effectiveness of Japanese traditional (Kampo) medicine is gradually attracting attention worldwide. It is typically known to be effective against cancer as a supportive therapy, but the antitumor effect of Kampo formulas is not fully established. We therefore evaluated the antitumor effect of eppikajutsuto.Methods: We evaluated the antitumor effect of eppikajutsuto by cell viability assay, western blotting, and apoptotic cell assay using an oral squamous cell carcinoma cell line OSC-19.Results: We found that the administration of eppikajutsuto to OSC-19 in vitro inhibited cell proliferation, induced apoptosis, and suppressed mTOR activation.Conclusions: The results suggested that eppikajutsuto might exhibit antitumor effect against OSC-19 in vitro by suppressing mTOR activation. However, additional studies in vitro and in vivo are required to confirm the antitumor effect of eppikajutsuto.