Enhanced surfactant protein and defensin mRNA levels and reduced viral replication during parainfluenza virus type 3 pneumonia in neonatal lambs
Enhanced surfactant protein and defensin mRNA levels and reduced viral replication during parainfluenza virus type 3 pneumonia in neonatal lambs
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DOI：
10.1128/cdli.11.3.599-607.2004
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发表时间：
2004-05-01
期刊：
影响因子：
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通讯作者：
Ackermann, MR
中科院分区：
文献类型：
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作者：
Grubor, B;Gallup, JM;Ackermann, MR
防御素和表面活性蛋白A（SP-A）和SP-D是肺先天免疫系统的抗微生物组分。本研究的目的是确定新生羔羊中副流感病毒3型感染改变绵羊β-防御素1（SBD-1）、SP-A和SP-D表达的程度，所有这些蛋白均由呼吸道上皮组成型转录。免疫组化（IHC）检测到副流感病毒3型病毒抗原在所有感染羔羊的细支气管接种后3天，接种后6天，在减少的水平，但它是不存在的接种后17天。在接种后的所有时间，通过荧光实时逆转录酶PCR检测，接种3型副流感病毒动物的肺匀浆中SBD-1、SP-A和SP-D mRNA水平均升高。定量竞争酶联免疫吸附试验检测肺组织匀浆中SP-A蛋白水平和免疫组化检测SP-A蛋白抗原均未发生改变。这些研究表明，3型副流感病毒感染导致呼吸道上皮细胞表达的组成型转录的先天免疫因子的表达增强，并且这种表达增加与病毒复制减少同时发生。
Defensins and surfactant protein A (SP-A) and SP-D are antimicrobial components of the pulmonary innate immune system. The purpose of this study was to determine the extent to which parainfluenza type 3 virus infection in neonatal lambs alters expression of sheep beta-defensin 1 (SBD-1), SP-A, and SP-D, all of which are constitutively transcribed by respiratory epithelia. Parainfluenza type 3 viral antigen was detected by immunohistochemistry (IHC) in the bronchioles of all infected lambs 3 days postinoculation and at diminished levels 6 days postinoculation, but it was absent 17 days postinoculation. At all times postinoculation, lung homogenates from parainfluenza type 3 virus-inoculated animals had increased SBD-1, SP-A, and SP-D mRNA levels as detected by fluorogenic real-time reverse transcriptase PCR. Protein levels of SP-A in lung homogenates detected by quantitative-competitive enzyme-linked immunosorbent assay and protein antigen of SP-A detected by IHC were not altered. These studies demonstrate that parainfluenza type 3 virus infection results in enhanced expression of constitutively transcribed innate immune factors expressed by respiratory epithelia and that this increased expression occurs concurrently with decreased viral replication.