Effect of astaxanthin on human sperm capacitation.
Effect of astaxanthin on human sperm capacitation.
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DOI：
10.3390/md11061909
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发表时间：
2013-06-03
期刊：
影响因子：
5.4
通讯作者：
Bordin L
中科院分区：
文献类型：
--
作者：
Donà G;Kožuh I;Brunati AM;Andrisani A;Ambrosini G;Bonanni G;Ragazzi E;Armanini D;Clari G;Bordin L
为了能够使卵母细胞受精，人类精子必须经历一系列形态和结构的改变，称为获能。研究表明，精子内源性活性氧（ROS）的产生在导致细胞发生大量顶体反应（AR）中起着关键作用。虾青素（Asta）是属于类胡萝卜素家族的光保护性红色素，被认为具有抗氧化、抗癌、抗糖尿病和抗炎特性，并且存在于许多膳食补充剂中。本研究评估了Asta在获能缓冲液中的作用，该缓冲液诱导低ROS产生和低百分比的顶体反应细胞（ARC）。在存在或不存在浓度递增的Asta或二酰胺（Diam）的情况下孵育精子细胞，并分析其ROS产生、Tyr-磷酸化（Tyr-P）模式以及ARC和非活细胞（NVC）的百分比。结果表明，Asta改善了精子头部Tyr-P和ARC值，而不影响ROS生成曲线，而Diam成功地提高了Tyr-P水平，但仅提高了鞭毛的水平，而不提高ARC值。这表明Asta可以插入膜中，因此产生容化样膜改变，从而允许Tyr-P的头部。一旦发生这种情况，AR就可以发生，并涉及更多数量的细胞。
In order to be able to fertilize oocytes, human sperm must undergo a series of morphological and structural alterations, known as capacitation. It has been shown that the production of endogenous sperm reactive oxygen species (ROS) plays a key role in causing cells to undergo a massive acrosome reaction (AR). Astaxanthin (Asta), a photo-protective red pigment belonging to the carotenoid family, is recognized as having anti-oxidant, anti-cancer, anti-diabetic and anti-inflammatory properties and is present in many dietary supplements. This study evaluates the effect of Asta in a capacitating buffer which induces low ROS production and low percentages of acrosome-reacted cells (ARC). Sperm cells were incubated in the presence or absence of increasing concentrations of Asta or diamide (Diam) and analyzed for their ROS production, Tyr-phosphorylation (Tyr-P) pattern and percentages of ARC and non-viable cells (NVC). Results show that Asta ameliorated both sperm head Tyr-P and ARC values without affecting the ROS generation curve, whereas Diam succeeded in enhancing the Tyr-P level but only of the flagellum without increasing ARC values. It is suggested that Asta can be inserted in the membrane and therefore create capacitation-like membrane alteration which allow Tyr-P of the head. Once this has occurred, AR can take place and involves a higher numbers of cells.
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