A worldwide survey of tomato yellow leaf curl viruses
A worldwide survey of tomato yellow leaf curl viruses
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DOI：
10.1007/s007050050168
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发表时间：
1997-01-01
影响因子：
2.7
通讯作者：
Laterrot, H
中科院分区：
文献类型：
--
作者：
Czosnek, H;Laterrot, H
番茄黄化卷叶病毒(TYLCV)是指影响热带和亚热带地区番茄栽培的几种粉虱传播的双生病毒。用来自以色列的TYLCV克隆分离物(TYLCV-ISR)的两个DNA探针进行杂交试验，以评估来自25个国家的自然感染的黄化卷叶或卷叶症状番茄植株中病毒的亲和力。探针A包括大部分基因间隔区，预计只能检测到与TYLCV-ISR密切相关的分离物，特别是在高强度洗涤之后。相比之下，包括全长基因组的探针B预计将检测到广泛的粉虱传播的双生病毒。来自中东六个国家、古巴或多米尼加共和国的番茄样本被证明与TYLCV-ISR密切相关，可能感染了相同的病毒株。塞内加尔和佛得角群岛的样本也与中东病毒有关。来自西地中海地区、非洲或东南亚其他九个国家的样本关系较远，可能代表一个或多个额外的双生病毒物种。来自非洲、中美洲或南美洲五个国家的样本只有在低严格洗涤后才给出与全长病毒基因组的杂交信号，表明这些样本被远程病毒感染。这些结果得到了DNA和蛋白质序列比较的支持，这表明番茄双生病毒属于三个主要的簇，代表来自1)地中海/中东/非洲地区，2)印度，远东和澳大利亚，以及3)美洲的病毒。在第一组病毒中，区分了来自地中海西部或中东/加勒比群岛的两个亚组病毒。1990-1996年间，番茄黄化曲叶病的发病率有较大幅度的增加。
The name tomato yellow leaf curl virus (TYLCV) has been given to several whitefly-transmitted geminiviruses affecting tomato cultures in many tropical and subtropical regions. Hybridization tests with two DNA probes derived from a cloned isolate of TYLCV from Israel (TYLCV-ISR) were used to assess the affinities of viruses in naturally infected tomato plants with yellow leaf curl or leaf curl symptoms from 25 countries. Probe A which included most of the intergenic region was expected to detect only isolates closely related to TYLCV-ISR, especially after high stringency washes. In contrast probe B, which included the full-length genome, was expected to detect a wide range of whitefly-transmitted geminiviruses. Tomato samples from six countries in the Middle East, from Cuba or the Dominican Republic proved to be closely related to TYLCV-ISR and probably were infected by strains of the same virus. Samples from Senegal and Cape Verde Islands were also related to the Middle Eastern virus. Samples from nine other countries in the western Mediterranean area, Africa, or South-East Asia were more distantly related and probably represent one or more additional geminivirus species. Samples from five countries in Africa, Central or South America gave hybridization signals with the full-length viral genome, only after low stringency wash, indicating that these samples were infected by remote viruses. These results were supported by DNA and protein sequence comparison, which indicate that tomato geminiviruses fall into three main clusters representing viruses from 1) the Mediterranean/Middle East/African region, 2) India, the Far East and Australia, and 3) the Americas. Within the first cluster, two sub-clusters of viruses from the western Mediterranean or from the Middle East/Caribbean Islands were distinguished. The incidence of tomato yellow leaf curl diseases has increased considerably between 1990 and 1996.