Three-dimensional macromolecular organization of cryofixed Myxococcus xanthus biofilms as revealed by electron microscopic tomography.
Three-dimensional macromolecular organization of cryofixed Myxococcus xanthus biofilms as revealed by electron microscopic tomography.
复制标题
电子显微镜断层扫描显示冷冻固定黄色粘球菌生物膜的三维大分子组织。
DOI：
10.1128/jb.01333-08
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发表时间：
2009
影响因子：
3.2
通讯作者：
Auer,Manfred
中科院分区：
文献类型：
--
作者：
Palsdottir,Hildur;Remis,JonathanP;Schaudinn,Christoph;O'Toole,Eileen;Lux,Renate;Shi,Wenyuan;McDonald,KentL;Costerton,JWilliam;Auer,Manfred
尽管大多数细菌生长在自然和致病生态系统的生物膜中，但人们对其组成细胞的超微结构或其群落结构的细节知之甚少。我们使用了高压冷冻和冷冻替代，以最大限度地减少化学固定、样品聚集和样品提取的伪影。作为进一步的创新，我们首次在生物膜研究中使用了电子断层扫描和三维(3D)可视化来更好地解析生物膜的大分子3D超微结构。这种精湛的标本制备和极大提高的轴向分辨率相结合，为研究黄色粘球菌的超微结构和生物膜群落结构打开了一扇窗。提出了有关生物膜基质中染色质小体、细胞质组织、相邻细胞之间的膜结合以及菌毛和囊泡的结构和分布的新的结构信息。
Despite the fact that most bacteria grow in biofilms in natural and pathogenic ecosystems, very little is known about the ultrastructure of their component cells or about the details of their community architecture. We used high-pressure freezing and freeze-substitution to minimize the artifacts of chemical fixation, sample aggregation, and sample extraction. As a further innovation we have, for the first time in biofilm research, used electron tomography and three-dimensional (3D) visualization to better resolve the macromolecular 3D ultrastructure of a biofilm. This combination of superb specimen preparation and greatly improved resolution in thezaxis has opened a window in studies ofMyxococcus xanthuscell ultrastructure and biofilm community architecture. New structural information on the chromatin body, cytoplasmic organization, membrane apposition between adjacent cells, and structure and distribution of pili and vesicles in the biofilm matrix is presented.