Enzyme-Powered Nanobots Enhance Anticancer Drug Delivery
Enzyme-Powered Nanobots Enhance Anticancer Drug Delivery
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DOI：
10.1002/adfm.201705086
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发表时间：
2018-06-20
影响因子：
19
通讯作者：
Sanchez, Samuel
中科院分区：
文献类型：
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作者：
Hortelao, Ana C.;Patino, Tania;Sanchez, Samuel
利用酶催化为利用生物相容性燃料的微型和纳米电机提供动力，为生物医学应用开辟了新的领域，例如将特定药物主动运输和递送到感兴趣的部位。在这里，介绍了用于阿霉素（Dox）抗癌药物装载、释放和有效递送至细胞的脲酶动力纳米马达（纳米机器人）。这些介孔硅基核壳纳米机器人能够在离子介质中自推进，如通过光学跟踪和动态光散射分析所证实的。与被动对应物相比，纳米机器人在6小时后实现了药物释放的四倍增加。此外，使用装载Dox的纳米机器人对HeLa细胞表现出增强的抗癌效率，这是由于增强的药物释放和在高浓度尿素底物下产生的氨的协同效应。与被动Dox负载的纳米颗粒相比，在与活性纳米机器人孵育1、4、6和24小时后检测到HeLa细胞内更高含量的Dox。酶动力纳米机器人实现的药物递送效率的提高可能会使其在未来的生物医学应用中具有潜力，例如在目标位置的基质触发药物释放。
The use of enzyme catalysis to power micro- and nanomotors exploiting biocompatible fuels has opened new ventures for biomedical applications such as the active transport and delivery of specific drugs to the site of interest. Here, urease-powered nanomotors (nanobots) for doxorubicin (Dox) anticancer drug loading, release, and efficient delivery to cells are presented. These mesoporous silica-based core-shell nanobots are able to self-propel in ionic media, as confirmed by optical tracking and dynamic light scattering analysis. A four-fold increase in drug release is achieved by nanobots after 6 h compared to their passive counterparts. Furthermore, the use of Dox-loaded nanobots presents an enhanced anticancer efficiency toward HeLa cells, which arises from a synergistic effect of the enhanced drug release and the ammonia produced at high concentrations of urea substrate. A higher content of Dox inside HeLa cells is detected after 1, 4, 6, and 24 h incubation with active nanobots compared to passive Dox-loaded nanoparticles. The improvement in drug delivery efficiency achieved by enzyme-powered nanobots may hold potential toward their use in future biomedical applications such as the substrate-triggered release of drugs in target locations.