A cloned ATP:guanidino kinase in the trematode Schistosoma mansoni has a novel duplicated structure.
A cloned ATP:guanidino kinase in the trematode Schistosoma mansoni has a novel duplicated structure.
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DOI：
10.1016/s0021-9258(19)39187-2
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发表时间：
1990-04
期刊：
影响因子：
--
通讯作者：
L. Stein;D. Harn;J. David
中科院分区：
文献类型：
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作者：
L. Stein;D. Harn;J. David
肌酸激酶(CK)是一种保守的ATP酶家族成员，其成员在细胞内的能量流动中起着重要作用。该家族以前的特征是由两个相关的40 kDa亚基组成的大约80 kDa的二聚体。我们克隆了曼氏血吸虫寄生吸虫的一个基因，该基因与CK有很大的氨基酸序列相似性。与脊椎动物Cks的基因一样，该基因受发育调节；在感染性尾蚴阶段，mRNA水平很高，但在转化为寄生虫血吸虫阶段后，mRNA水平迅速下降。然而，与先前描述的胍基磷酸转移酶家族成员不同，血吸虫基因似乎是两个CK样结构域的直接融合，这两个结构域编码一个单一的74 kDa多肽。从粗制寄生虫匀浆的酶分析中得到的相关证据表明，克隆的基因是一种肌酸激酶。这是无脊椎动物三磷酸腺苷：胍基磷酸转移酶的首次分子克隆。
Creatine kinase (CK) is part of a conserved family of ATP:guanidino phosphotransferases whose members play important roles in intracellular energy flow. Previously characterized members of this family are approximately 80-kDa dimers of two related 40-kDa subunits. We have cloned a gene from the parasitic trematode Schistosoma mansoni which has substantial amino acid sequence similarities to CK. Like the genes for vertebrate CKs, this gene is developmentally regulated; mRNA levels are high in the infective cercarial stage but rapidly decrease upon transformation to the parasitic schistosomulum stage. In contrast to members of the guanidino phosphotransferase family characterized previously, however, the schistosome gene appears to be a direct fusion of two CK-like domains that encode a single 74-kDa polypeptide. Correlative evidence from enzyme assays of crude parasite homogenates suggests that the cloned gene is a creatine kinase. This represents the first molecular cloning of an invertebrate ATP:guanidino phosphotransferase.