Maternal separation affects Anxiety like behavior begin in adolescence continue through adulthood and related to Dnmt3a expression.
Maternal separation affects Anxiety like behavior begin in adolescence continue through adulthood and related to Dnmt3a expression.
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DOI：
10.1152/jn.00247.2022
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发表时间：
2022-08
影响因子：
2.5
通讯作者：
Xiaobing Wang;Le Jiang;Wenhao Ma;Xiaoye Zheng;Ershu He;Bensi Zhang;Manoj Kumar Vashisth;Zhiting Gong
中科院分区：
文献类型：
--
作者：
Xiaobing Wang;Le Jiang;Wenhao Ma;Xiaoye Zheng;Ershu He;Bensi Zhang;Manoj Kumar Vashisth;Zhiting Gong
早期生活压力，包括母亲分离，是青少年焦虑的主要原因之一。DNA甲基转移酶3A （Dnmt3a）是调节DNA甲基化的关键分子，被发现与焦虑样行为有关。目前尚不清楚母亲分离是否会影响不同发育阶段小鼠的焦虑水平，或者Dnmt3a是否在这一过程中发挥作用。本研究通过开放场实验探讨母分离对不同年龄小鼠类焦虑行为的影响，发现母分离能成功诱导青春期小鼠类焦虑行为，并持续到成年期。通过western blot，我们发现P17母分离小鼠海马和皮质Dnmt3a水平呈现不同的变化趋势。此外，通过免疫染色，我们发现Dnmt3a在皮层和海马中的表达水平有显著差异，并且随着小鼠年龄的增长有不同程度的下降，这是导致不同趋势的原因。我们的研究结果为进一步开发更适合青少年的焦虑/抑郁治疗方案提供了实验基础。
Early life stress, including maternal separation, is among one of the main causes of anxiety in adolescents. DNA methyltransferase 3A (Dnmt3a) is a key molecule that regulates DNA methylation and is found to be associated with anxiety-like behavior. It is not clear whether maternal separation affects anxiety levels in mice at different developmental stages, or whether Dnmt3a plays a role in this process. Here, by using open field test to exploring the effect of maternal separation on anxiety-like behavior in mice of different age, it was found that maternal separation could successfully induce anxiety-like behavior in adolescent mice, and which continued through adulthood. By using western blot, we found the levels of Dnmt3a in the hippocampus and cortex have shown different trends in maternal separation mice on P17. Further, by using immunostaining, we have found that the expression levels of Dnmt3a in the cortex and hippocampus were significantly different, and decreased to varying degrees with the age of mice, which being the reason for different trends. Our results provide an experimental basis for further development of anxiety/depression treatment programs more suitable for adolescence.