D-Allulose is a substrate of glucose transporter type 5 (GLUT5) in the small intestine
D-Allulose is a substrate of glucose transporter type 5 (GLUT5) in the small intestine
复制标题
DOI：
10.1016/j.foodchem.2018.11.003
复制
发表时间：
2019-03-30
期刊：
影响因子：
8.8
通讯作者：
Toyoda, Yukiyasu
中科院分区：
文献类型：
--
作者：
Kishida, Kunihiro;Martinez, Gustavo;Toyoda, Yukiyasu
据报道，D-Allulose对健康有益。然而，介导肠道D-allulose运输的运输系统尚未被清楚地确定。本研究的目的是探讨肠道D-allulose转运是否由葡萄糖转运蛋白5型（GLUT5）介导。当单独灌胃D-allulose时，先前喂食果糖的大鼠血浆D-allulose水平显著升高。这表明肠道D-allulose吸收的增强与GLUT5表达的增加平行，仅在果糖喂养的大鼠中观察到。当D-allulose被d -果糖灌胃时，先前观察到的血浆D-allulose水平的增加被抑制和延迟，这表明d -果糖抑制了D-allulose经上皮向血浆的转运。示踪剂D[C-14]-果糖的摄取率在50mM D-果糖中降低到54.8%，在50mM D-果糖中降低到16.4%，表明D-allulose与D-[C-14]-果糖竞争，D-allulose对GLUT5的亲和力低于D-果糖。GLUT5明显介导肠道D-allulose运输，可能相对于d -果糖的亲和力较低。
D-Allulose has been reported to have beneficial health effects. However, the transport system(s) mediating intestinal D-allulose transport has not yet been clearly identified. The aim of this study was to investigate whether intestinal D-allulose transport is mediated by glucose transporter type 5 (GLUT5). When D-allulose alone was gavaged, plasma D-allulose levels were dramatically higher in rats previously fed fructose. This suggests enhanced intestinal D-allulose absorption paralleled increases in GLUT5 expression observed only in fructose-fed rats. When D-allulose was gavaged with D-fructose, previously observed increases in plasma D-allulose levels were dampened and delayed, indicating D-fructose inhibited transepithelial D-allulose transport into plasma. Tracer D[C-14]-fructose uptake rate was reduced to 54.8% in 50mM D-allulose and to 16.4% in 50mM D-fructose, suggesting D-allulose competed with D-[C-14]-fructose and the affinity of D-allulose for GLUT5 was lower than that of D-fructose. GLUT5 clearly mediates, likely at lower affinity relative to D-fructose, intestinal D-allulose transport.