Ag-loaded mesoporous bioactive glasses against Enterococcus faecalis biofilm in root canal of human teeth
Ag-loaded mesoporous bioactive glasses against Enterococcus faecalis biofilm in root canal of human teeth
复制标题
载银介孔生物活性玻璃可对抗人牙根管内粪肠球菌生物膜。
DOI：
10.4012/dmj.2014-104
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发表时间：
2015-02-01
影响因子：
2.5
通讯作者：
Fan, Bing
中科院分区：
文献类型：
--
作者：
Fan, Wei;Wu, Daming;Fan, Bing
制备了载银介孔生物活性玻璃（Ag-MBG）粉体，并对其进行了表征。测定了银微球在Tris-HCl溶液中的离子释放量和浸泡后模拟体液的pH稳定性。用粪肠球菌（Enterococcus faecalis）接种根管4周，观察MBGs、Ag-MBGs和氢氧化钙对粪肠球菌（Enterococcus faecalis）的抗菌活性。粪便生物膜进行评价。结果表明，Ag-MBGs具有高度有序的介孔结构，银纳米粒子沉积在介孔中，使银离子得以持续释放。与MBGs相比，用Ag-MBGs处理的生物膜显示出显著的结构破坏。这些结果表明，银微球对根管内粪肠球菌生物膜具有较强的抗菌作用，其抗菌活性是通过银离子的释放而产生的。
Ag-loaded mesoporous bioactive glass (Ag-MBG) powders were synthesized and characterized. The ions release of Ag-MBGs in Tris-HCl and the pH stability of simulated body fluids after immersing Ag-MBGs were tested. Root canals were inoculated with Enterococcus faecalis for 4 weeks, and the antibacterial activity of MBGs, Ag-MBGs and calcium hydroxide against E. faecalis biofilm were evaluated. Results showed that Ag-MBGs possessed highly ordered mesoporous structure with silver nanoparticles deposited in the mesopores, which enabled a sustained Ag ions released. The biofilms treated with Ag-MBGs showed a significant structural disruption compared with MBGs. These results indicated that Ag-MBGs possess a potent antibacterial effect against E.faecalis biofilm in root canal, and the antibacterial activity was induced by the release of Ag ions from Ag-MBGs.