Identification of host proteins involved in rickettsial invasion of tick cells.
Identification of host proteins involved in rickettsial invasion of tick cells.
复制标题
鉴定参与立克次体入侵蜱细胞的宿主蛋白。
DOI：
10.1128/iai.02888-14
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发表时间：
2015
影响因子：
3.1
通讯作者：
Macaluso,KevinR
中科院分区：
文献类型：
--
作者：
Petchampai,Natthida;Sunyakumthorn,Piyanate;Banajee,KaikhushrooH;Verhoeve,VictoriaI;Kearney,MichaelT;Macaluso,KevinR
蜱传斑点热群（SFG）立克次体是能够感染脊椎动物和无脊椎动物宿主细胞的专性细胞内细菌，这是随后细菌在不同宿主中存活的必要过程。已经鉴定了参与立克次体进入哺乳动物和果蝇细胞的宿主细胞信号分子;然而，对侵入蜱细胞的研究不足。考虑到SFG立克次体在脊椎动物和无脊椎动物宿主之间的移动，假设是保守的机制被用于宿主细胞入侵。目前的研究采用生化抑制试验，以确定蜱蛋白参与立克次体感染蜱源性细胞从自然宿主，Dermacentor variabilis。结果显示，立克次体侵袭过程中起重要作用的蛋白质包括肌动蛋白丝、肌动蛋白相关蛋白2/3复合物、磷脂酰肌醇-3 ′-激酶、蛋白酪氨酸激酶（PTKs）、Src家族PTK、黏着斑激酶、Rho GTdR Rac 1和神经Wiskott-Aldrich综合征蛋白。阐明立克次体感染的分子机制对于深入了解立克次体在蜱虫种群中的传播和蜱传立克次体病的生态学至关重要。
Tick-borne spotted fever group (SFG) Rickettsia species are obligate intracellular bacteria capable of infecting both vertebrate and invertebrate host cells, an essential process for subsequent bacterial survival in distinct hosts. The host cell signaling molecules involved in the uptake of Rickettsia into mammalian and Drosophila cells have been identified; however, invasion into tick cells is understudied. Considering the movement of SFG Rickettsia between vertebrate and invertebrate hosts, the hypothesis is that conserved mechanisms are utilized for host cell invasion. The current study employed biochemical inhibition assays to determine the tick proteins involved in Rickettsia montanensis infection of tick-derived cells from a natural host, Dermacentor variabilis. The results revealed several tick proteins important for rickettsial invasion, including actin filaments, actin-related protein 2/3 complex, phosphatidylinositol-3′-kinase, protein tyrosine kinases (PTKs), Src family PTK, focal adhesion kinase, Rho GTPase Rac1, and neural Wiskott-Aldrich syndrome protein. Delineating the molecular mechanisms of rickettsial infection is critical to a thorough understanding of rickettsial transmission in tick populations and the ecology of tick-borne rickettsial diseases.