The Effect of Growth Temperature on the Pathogenicity of Campylobacter
The Effect of Growth Temperature on the Pathogenicity of Campylobacter
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DOI：
10.1007/s00284-013-0370-1
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发表时间：
2013-09-01
影响因子：
2.6
通讯作者：
Humphrey, Tom J.
中科院分区：
文献类型：
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作者：
Aroori, Sree V.;Cogan, Tristan A.;Humphrey, Tom J.
控制食物链中的弯曲杆菌需要更好地了解细菌在相关环境中的行为。弯曲杆菌属物种在家禽中基本上是非致病性的，其体温为42 A摄氏度。然而，这种细菌对体温为37 A摄氏度的人具有高致病性。本研究的目的是检查是否转换从寄生到致病行为与温度有关。本实验研究了三种弯曲杆菌对T84细胞的生长、运动和侵袭能力的影响。jejuni81116、空肠弯曲菌C. jejuni M1、C. coli1669、C. coliRM2228和C.胎儿NC 10842在37和42 A ℃下生长。我们的结果表明C.空肠分离株在这两种温度下生长相似，但有些在42 ℃下更具运动性，有些在37 ℃下更具侵袭性，这可能分别解释了其在家禽群中的快速传播和在人类中的感染。C.大肠杆菌是人类弯曲杆菌感染的罕见原因，与42 A摄氏度相比，在37 A摄氏度下生长和移动的能力较低，但在较低温度下更具侵袭性。C.在家禽中很少发现的胎儿，在42摄氏度时生长和侵入的能力较低。
Control of Campylobacter in the food chain requires a better understanding of the behaviour of the bacteria in relevant environments. Campylobacter species are largely non-pathogenic in poultry, the body temperature of which is 42 A degrees C. However, the bacteria are highly pathogenic in humans whose body temperature is 37 A degrees C. The aim of this study was to examine if switching from commensal to pathogenic behaviour was related to temperature. We examined the growth, motility and invasion of T84 cells by three species of Campylobacter: C. jejuni 81116, C. jejuni M1, C. coli 1669, C. coli RM2228 and C. fetus fetus NC10842 grown at 37 and 42 A degrees C. Our results suggest that C. jejuni isolates grow similarly at both temperatures but some are more motile at 42 A degrees C and some are more invasive at 37 A degrees C, which may account for its rapid spread in poultry flocks and for infection in humans, respectively. C. coli, which are infrequent causes of Campylobacter infections in humans, is less able to grow and move at 37 A degrees C compared to 42 A degrees C but was significantly more invasive at the lower temperature. C. fetus fetus, which is infrequently found in poultry, is less able to grow and invade at 42 A degrees C.