Neuropeptide S reduces propofol- or ketamine-induced slow wave states through activation of cognate receptors in the rat
Neuropeptide S reduces propofol- or ketamine-induced slow wave states through activation of cognate receptors in the rat
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Neuropeptide S 通过激活大鼠中的同源受体来减少丙泊酚或氯胺酮诱导的慢波状态
DOI：
10.1016/j.npep.2017.02.081
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发表时间：
2017-06-01
期刊：
影响因子：
2.9
通讯作者：
Hou, Yi-Ping
中科院分区：
文献类型：
--
作者：
Kong, Xiang-Pan;Wang, Can;Hou, Yi-Ping
脑室注射NPS可缩短氯胺酮或硫喷妥钠引起的大鼠翻正反射消失的持续时间。但具体的脑电活动尚不清楚。因此，我们试图研究NPS-NPSR系统对麻醉诱导的脑电功率谱和睡眠-觉醒曲线特征的影响。NPS单独或与NPSR拮抗剂一起侧脑室注射，异丙酚(50 mg/kg)或氯胺酮(100 mg/kg)腹腔注射。NPS(1或2nmol)可显著降低异丙酚诱发的脑电增量活动和慢波状态(SWS)。NPS(1或5nmol)可显著降低氯胺酮诱发的短波反射和EEG Delta活动。皮层脑电功率谱分析显示，异丙酚可显著增加盐水组大鼠的Delta值(0.5~4 Hz)，降低theta值(4.5~8.5 Hz值)，降低高频值(14.5~60 Hz值)，而1nmolNPS组大鼠Delta值持续时间缩短，频谱模式无明显改变。而盐水组注射氯胺酮后，β波(4.5~8.5赫兹)波活动明显增加，而高频波(14.5~60赫兹)波活动明显降低。然而，在1nmoL NPS预处理下，Delta活性降低，theta活性增加。NPS对麻醉性SWS的抑制作用以缩短SWS发作时程为特征，对SWS发作次数和潜伏期无明显影响。NPSR拮抗剂[D-Val(5)]NPS(20nmoL)可显著减弱1nmolNPS的促醒作用，但对SWS无明显影响。我们推测，NPS通过选择性地激活NPSR，从而触发随后的觉醒通路，从而显著减少麻醉诱导的SWS和EEG慢活动。(C)2017爱思唯尔有限公司。保留所有权利。
Intracerebroventricular injection of NPS reduces the duration of the ketamine- or thiopental-induced loss of the righting reflex in rats. But the specific EEG activities are unknown. We therefore sought to examine the effects of the NPS-NPSR system on anesthetic-induced characteristics of EEG power spectra and sleep-wake profiles. NPS alone or together with an NPSR antagonist was injected intracerebroventricularly, whereas the propofol (50 mg/kg) or ketamine (100 mg/kg) was administrated intraperitoneally. NPS (1 or 2 nmol) significantly reduced the amount of propofol-induced EEG delta activity and slow wave states (SWS). NPS (1 or 5 nmol) significantly reduced the amount of ketamine-induced SWS and EEG delta activity. Cortical EEG power spectral analysis showed that, in saline-pretreated rats, propofol induced a marked increase in delta (0.5-4 Hz) activity, decrease in theta (4.5-8.5 Hz) activity, and decrease in high frequency activity (14.5-60 Hz), while, in rats pretreated with 1 nmol of NPS, the duration of delta activity was reduced, while its spectral pattern was not changed. Whereas injection of ketamine into saline -pretreated rats induced a marked increase in delta (0.54 Hz) activity, a moderate increase in theta (4.5-8.5 Hz) activity, and a marked decrease in high frequency (14.5-60 Hz) activity. However, delta activity was reduced while theta activity increased under pretreatment with 1 nmol of NPS. The inhibitory effect of NPS on anesthetic-induced SWS was characterized by a reduced SWS episode duration with no significant change in either episode number or latency to SWS. [D-Val(5)]NPS, an NPSR antagonist (20 nmol), significantly attenuated the arousal-promoting effect of 1 nmol of NPS, but had no effect on SWS when injected alone. We speculate that NPS significantly reduces anesthetic-induced SWS and EEG slow activity by selective activation of the NPSR, which, in turn, would trigger subsequent arousal pathways. (C) 2017 Elsevier Ltd. All rights reserved.