The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells.
The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells.
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Ly-49 家族：调节小鼠 CD3 细胞的细胞毒性和细胞因子产生。
作者：
J. Ortaldo;R. Winkler;A. Mason;L. Mason
Ly-49基因家族是鼠NK细胞上的I类识别受体。大多数Ly-49受体在识别其靶I类配体后抑制NK细胞溶解。在本报告中，我们研究了Ly-49 A和Ly-49 G2调节CD 3+细胞上的T细胞功能的能力，主要是也表达NK-1.1和/或DX 5的亚群。大多数（>50%）表达Ly-49分子的T细胞也共表达NK-1.1和/或DX 5，尽管一些NK-1.1-和/或DX 5-/CD 3+细胞表达Ly-49分子。通过表达Ly-49 A和G2的IL-2培养的T细胞对靶细胞的裂解被Ly-49 A和G2特异性的Ab以及I类（H-2Dd α 1/α 2）的Ab增强。鼠T细胞也在表达（H-2Dd）的靶的存在下培养，所述靶抑制Ly-49 A和G2受体。检查这些细胞的细胞因子产生（IFN-γ、TNF-α和粒细胞-巨噬细胞CSF）的同时增加。针对Ly-49 A和G2或它们各自的I类配体的抗体阻断了通过Ly-49受体介导的负信号，并在这些T细胞与表达H-2Dd的肿瘤靶相互作用后增加了IFN-γ和粒细胞-巨噬细胞CSF的产生。此外，当用mAb YE 148和4D 11处理时，表达Ly-49 A和G2的EL-4 T细胞系表现出减少的细胞因子产生和钙动员。这些结果首次证明，Ly-49 I类结合受体，以前被认为是局限于小鼠NK细胞，可以介导T细胞亚群的重要生理功能。
The Ly-49 gene families are class I-recognizing receptors on murine NK cells. Most Ly-49 receptors inhibit NK cell lysis upon recognizing their target class I ligands. In this report we have examined the ability of Ly-49A and Ly-49G2 to regulate T cell functions on CD3+ cells, primarily the subset that also expresses NK-1.1 and/or DX5. The majority (>50%) of T cells that express Ly-49 molecules also coexpress NK-1.1 and/or DX5, although some NK-1.1- and/or DX5-/CD3+ cells express Ly-49 molecules. Lysis of target cells by IL-2-cultured T cells expressing Ly-49A and G2 was enhanced by Abs specific for Ly-49A and G2 as well as by Abs to class I (H-2Dd alpha1/alpha2). Murine T cells also were cultured in the presence of targets that express (H-2Dd) which is inhibiting for the Ly-49A and G2 receptors. These cells were examined for a coincident increase in cytokine production (IFN-gamma, TNF-alpha, and granulocyte-macrophage CSF). Abs to Ly-49A and G2 or their respective class I ligands blocked the negative signals mediated via the Ly-49 receptors and increased IFN-gamma and granulocyte-macrophage CSF production after interaction of these T cells with H-2Dd-expressing tumor targets. Furthermore, an EL-4 T cell line expressing both Ly-49A and G2, when treated with mAb YE148 and 4D11, demonstrated reduced cytokine production and calcium mobilization. These results demonstrate for the first time that Ly-49 class I binding receptors, previously thought to be restricted to mouse NK cells, can mediate important physiological functions of T cell subsets.
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DOI：
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发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
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发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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DOI：
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发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
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