Evaluation of humoral and cellular immune responses to BP26 and OMP31 epitopes in the attenuated Brucella melitensis vaccinated sheep
Evaluation of humoral and cellular immune responses to BP26 and OMP31 epitopes in the attenuated Brucella melitensis vaccinated sheep
复制标题
布鲁氏菌减毒疫苗接种绵羊对 BP26 和 OMP31 表位的体液和细胞免疫反应评估
DOI：
10.1016/j.vaccine.2013.12.028
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发表时间：
2014-02-07
期刊：
影响因子：
5.5
通讯作者：
Li, Chengyao
中科院分区：
文献类型：
--
作者：
Wang, Wenjing;Wu, Jingbo;Li, Chengyao
近年来，我国人间布鲁氏菌病病例数以每年约10%的速度递增。大多数病例是通过接触受感染的绵羊、山羊或其产品由羊种布鲁氏菌引起的。一种减弱的B。在中国，羊种球蛋白疫苗M5-90目前用于接种这两种动物。尚未研究该疫苗的关键参数，如免疫应答及其与保护效力的相关性。本研究以中国美利奴羊和哈萨克羊M5-90为免疫原，研究了外周质蛋白BP 26和外膜蛋白OMP 31对绵羊体液和细胞免疫应答的影响。检测到低水平的BP 26或OMP 31抗体，并定量特异性IFN-γ应答。来自BP 26和OMP 31的强反应性肽鉴定了五个T细胞表位（BP 26 -6、-8、-11、-12和OMP 31 -23），5个种属特异性表位（BP 26 -10、-18、-21和-22和OMP 31 -12）和四种动物特异性表位（BP 26 -15、-23、OMP 31 -6和-21），其刺激免疫绵羊中的特异性IFN-γ应答。在这些T细胞表位中，对BP 26 -18和-21表位的反应性与MHC-I B等位基因显著相关（P=0.024）。然而，M5-90疫苗诱导的特异性T细胞反应相对较弱，并且持续时间不够长，这可能会被疫苗接种后T调节细胞（Treg）的快速激活所抑制。这些发现为设计一种更安全，更有效的疫苗用于动物和人类提供了见解。(C)2013爱思唯尔有限公司保留所有权利。
In recent years, the number of cases of human brucellosis has been increasing by approximately 10% per year in China. Most cases were caused by Brucella melitensis through contacts with infected sheep, goats or their products. An attenuated B. melitensis vaccine M5-90 is currently used to vaccinate both animals in China. This vaccine has not been investigated for critical parameters such as immune response and its association with protective efficacy. In this study, humoral and cellular immune response to the periplasmic protein BP26 and the outer membrane protein OMP31 were evaluated in M5-90 vaccinated Chinese merino and Kazak sheep. Antibodies to BP26 or OMP31 were detected at low levels, and specific IFN-gamma response was quantified. Strongly reactive peptides derived from BP26 and OMP31 identified five T-cell epitopes (BP26-6, -8, -11, -12 and OMP31-23) common to both sheep species, five species-specific epitopes (BP26-10, -18, -21 and -22 and OMP31-12) and four animal-specific epitopes (BP26-15, -23, OMP31-6 and -21), which stimulated specific IFN-gamma response in vaccinated sheep. Among those T-cell epitopes, reactivity to BP26-18 and -21 epitopes was significantly associated with MHC-I B allele (P=0.024). However, a specific T-cell response induced by the M5-90 vaccine was relatively week and did not sustain long enough, which might be suppressed by rapid activation of T-regulatory (Treg) cells following vaccination. These findings provide an insight in designing a safer and more effective vaccine for use in animals and in humans. (C) 2013 Elsevier Ltd. All rights reserved.