How a circadian clock adapts to seasonal decreases in temperature and day length
How a circadian clock adapts to seasonal decreases in temperature and day length
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DOI：
10.1016/s0896-6273(00)80834-x
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发表时间：
1999-09-01
期刊：
影响因子：
16.2
通讯作者：
Edery, I
中科院分区：
文献类型：
--
作者：
Majercak, J;Sidote, D;Edery, I
我们表明，在3'非翻译区的mRNA的温度敏感剪接事件从周期（每）基因起着重要的作用，在果蝇的昼夜节律钟如何适应季节性寒冷的日子（低温和短日照）。增强剪接的内含子在低温下推进每mRNA和蛋白质周期的稳态阶段，事件显着有助于优先白天活动的苍蝇在寒冷的日子。由于PER的积累也依赖于光敏TIMELESS（TIM）蛋白，长的光周期通过延迟TIM水平的每日增加而部分抵消了振荡机制中冷诱导的进展。我们的研究结果还表明，有一个温度依赖性开关的分子逻辑控制周期在每mRNA水平。
We show that a thermosensitive splicing event in the 3' untranslated region of the mRNA from the period (per) gene plays an important role in how a circadian clock in Drosophila adapts to seasonally cold days (low temperatures and short day lengths). The enhanced splicing of this intron at low temperatures advances the steady state phases of the per mRNA and protein cycles, events that significantly contribute to the preferential daytime activity of flies on cold days. Because the accumulation of PER is also dependent on the photosensitive TIMELESS (TIM) protein, long photoperiods partially counteract the cold-induced advances in the oscillatory mechanism by delaying the daily increases in the levels of TIM. Our findings also indicate that there is a temperature-dependent switch in the molecular logic governing cycles in per mRNA levels.