Analysis of human plasma proteome by 2DE- and 2D nanoLC-based mass spectrometry.
Analysis of human plasma proteome by 2DE- and 2D nanoLC-based mass spectrometry.
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通过基于 2DE 和 2D nanoLC 的质谱法分析人血浆蛋白质组。
DOI：
10.1080/10826060500388447
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Park,JongHoon
中科院分区：
文献类型：
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作者：
Choi,Kyoung-Soo;Song,Liguo;Park,Young-Mee;Marshall,James;Lund,AndersL;Shion,Henry;Park,Eun-Mi;Chae,Ho-Zoon;Park,JongHoon
我们比较了2DE耦合MALDI-TOF-MS和ESI-MS/MS分析（2DE-MS）和在线2D nanoLC，然后进行nanoESI-MS/MS分析（2DLC-MS），用于分离和鉴定高丰度蛋白质耗尽的人血浆中的蛋白质。通过两种方法鉴定血浆中的蛋白质，证明大多数鉴定的蛋白质组对于每种方法是独特的。因此，如果需要蛋白质组鉴定的全面覆盖，则理想的是应用两种方法。2DE-MS方法适用于蛋白质斑点定量，而2DLC-MS方法可提供高通量应用的优势。
We compared the 2DE coupled to MALDI‐TOF‐MS and ESI‐MS/MS analysis (2DE‐MS) and the on‐line 2D nanoLC, followed by nanoESI‐MS/MS analysis (2DLC‐MS), for the separation and identification of proteins in high abundance protein‐depleted human plasma. Identification of proteins in the plasma by the two methods demonstrated that the majority of the identified protein set was unique to each method. Therefore, if a comprehensive coverage of the proteome identification is desired, it is ideal to apply both methods. The 2DE‐MS method is amenable to protein spot‐based quantitation, whereas the 2DLC‐MS method may provide an advantage of the high throughput application.