Butyrate and glucose metabolism by colonocytes in experimental colitis in mice
Butyrate and glucose metabolism by colonocytes in experimental colitis in mice
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DOI：
10.1136/gut.46.4.493
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发表时间：
2000-04-01
期刊：
影响因子：
24.5
通讯作者：
Murthy, SN
中科院分区：
文献类型：
--
作者：
Ahmad, MS;Krishnan, S;Murthy, SN
背景/目的：丁酸盐的结肠细胞代谢受损与溃疡性结肠炎的发病机制有关。结肠细胞丁酸盐代谢的影响，在实验性结肠炎mice. Methods结肠炎诱导瑞士远系白色小鼠口服给药的4%葡聚糖硫酸钠（DSS）。将从结肠炎小鼠和正常对照小鼠分离的结肠细胞与[C-14]丁酸盐或葡萄糖孵育，并测量（CO2）-C-14以及中间代谢物（乙酰乙酸盐、β-羟基丁酸盐和乳酸盐）的产生。结果-丁酸氧化（μ mol/h/mg蛋白;平均值（SEM））在DSS结肠炎中显著降低，DSS给药第7天的值为0.177（0.007），而对照动物为0.406（0.035）（p
Background/aims-Impaired colonocyte metabolism of butyrate has been implicated in the aetiopathogenesis of ulcerative colitis. Colonocyte butyrate metabolism was investigated in experimental colitis in mice.Methods-Colitis was induced in Swiss outbred white mice by oral administration of 4% dextran sulphate sodium (DSS). Colonocytes isolated from colitic and normal control mice were incubated with [C-14]butyrate or glucose, and production of (CO2)-C-14, as well as of intermediate metabolites (acetoacetate, beta-hydroxybutyrate and lactate), was measured. The effect of different substrate concentrations on oxidation was also examined.Results-Butyrate oxidation (mu mol/h per mg protein; mean (SEM)) was significantly reduced in DSS colitis, values on day 7 of DSS administration being 0.177 (0.007) compared with 0.406 (0.035) for control animals (p