Organohalogens and their hydroxylated metabolites in the blood of pigs from an open waste dumping site in south India: Association with hepatic cytochrome P450
Organohalogens and their hydroxylated metabolites in the blood of pigs from an open waste dumping site in south India: Association with hepatic cytochrome P450
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DOI：
10.1016/j.envres.2015.02.012
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发表时间：
2015-04-01
影响因子：
8.3
通讯作者：
Tanabe, Shinsuke
中科院分区：
文献类型：
--
作者：
Mizukawa, Hazuki;Nomiyama, Kei;Tanabe, Shinsuke
测定了南印度城市垃圾露天倾倒场(DS)和参照场(RS)欧亚野猪(Sus Scrofa)血液中多氯联苯(PCbs)、多溴联苯醚(PBDEs)及其羟化代谢物(OH-PCbs和OH-PBDEs)的含量。我们发现，来自DS的雌性猪受到的羟基多氯联苯的污染比所有其他成年猪都要高。来自DS的仔猪体内的羟基多氯联苯浓度最高。此外，仔猪肝脏中的细胞色素P450(CYP)酶在肝脏中的表达水平高于母猪，提示细胞色素P450(CYP)酶参与了多氯联苯的代谢。羟基-多氯联苯的同系物分布因性别和采集地点的不同而不同，可能是因为个体猪之间I相酶和II相酶的表达水平不同，暴露来源的差异，以及母体多氯联苯的母体转移。肝脏细胞色素P1a的表达水平与4OH-CB107、4OH-CB162和4OH-CB187的血液浓度呈正相关，表明这些羟基-多氯联苯在猪肝脏中的形成依赖于CYP1A。我们发现羟基多氯联苯的血液浓度与甲状腺激素(TH)之间没有显著的相关性；然而，DS猪的甲状腺激素(T-4)水平低于RS猪。我们有限的数据集表明，诱导的CYP酶加速了外源物质和内源分子在猪体内的代谢。因此，除了母体化合物外，还应考虑羟基代谢物进入野生动物和生活在城市露天垃圾场及其周围的人类的风险。(C)2015 Elsevier Inc.保留所有权利。
The concentrations of polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and their hydroxylated metabolites (OH-PCBs and OH-PBDEs) were measured in the blood of Eurasian wild pigs (Sus scrofa) from a municipal waste open dumping site (DS) and a reference site (RS) in South India. We showed that contamination with OH-PCBs was higher in female pigs from the DS than in all other adult pigs. The highest OH-PCB concentrations were found in piglets from the DS. Moreover, the hepatic expression levels of CYP1A and CYP2B were higher in piglets than in their dam, implying metabolism of PCBs by cytochrome P450 (CYP) enzymes. The OH-PCB congener profiles differed according to sex and collection sites, possibly because of variations in the expression levels of phase I and phase II enzymes among individual pigs, differences in the exposure sources, and maternal transfer of parent PCBs. The hepatic CYP1A expression levels were positively correlated with the blood concentrations of 4OH-CB107, 4OH-CB162, and 4OH-CB187, implying CYP1A-dependent formation of these OH-PCBs in the pig liver. We found no significant correlations between the blood concentrations of OH-PCBs and thyroid hormones (THs); however, the thyroxin (T-4) levels were lower in pigs from the DS than in pigs from the RS. Our limited dataset suggest that induced CYP enzymes accelerate the metabolism of xenobiotics and endogenous molecules in pigs. Thus, besides parental compounds, the risk of hydroxylated metabolites entering wildlife and humans living in and around municipal open waste dumping sites should be considered. (C) 2015 Elsevier Inc. All rights reserved.