Binding of glucan-binding protein C to GTFD-synthesized soluble glucan in sucrose-dependent adhesion of Streptococcus mutans
Binding of glucan-binding protein C to GTFD-synthesized soluble glucan in sucrose-dependent adhesion of Streptococcus mutans
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DOI：
10.1111/j.1399-302x.2005.00252.x
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发表时间：
2006-02-01
影响因子：
--
通讯作者：
Ooshima, T
中科院分区：
文献类型：
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作者：
Matsumoto, M;Fujita, K;Ooshima, T
变形链球菌产生葡聚糖结合蛋白（Gbp蛋白），其促进生物体与牙齿的粘附。已经鉴定了三种Gbp蛋白，GbpA蛋白、GbpB蛋白和GbpC蛋白;然而，葡聚糖与细菌细胞表面之间的粘附机制尚不清楚。我们使用葡萄糖基转移酶（GTF）和/或Gbp缺陷突变体来研究GbpC蛋白在S.玻璃表面的变形菌。野生型菌株MT 8148和GbpA缺陷型突变菌株显示出在添加rGTFD后增加的蔗糖依赖性粘附。然而，一个GbpC缺陷型突变株表现出蔗糖依赖性粘附的水平没有变化，尽管添加rGTFD。此外，rGbpC蛋白与rGTFD合成的葡聚糖的结合显著高于与rGTFB或rGTFC合成的葡聚糖的结合。这些结果表明，GbpC蛋白可能发挥了重要作用，蔗糖依赖性的粘附结合到GTFD合成的可溶性葡聚糖。
Streptococcus mutans produces glucan-binding proteins (Gbp proteins) which promote the adhesion of the organism to teeth. Three Gbp proteins, GbpA protein, GbpB protein, and GbpC protein have been identified; however, the mechanism of adhesion between glucans and bacterial cell surfaces is unknown. We used glucosyltransferase (GTF)- and/or Gbp-deficient mutants to examine the role of GbpC protein in the sucrose-dependent cellular adhesion of S. mutans to glass surfaces. The wild-type strain MT8148 and a GbpA-deficient mutant strain displayed increased sucrose-dependent adhesion following the addition of rGTFD. However, a GbpC-deficient mutant strain demonstrated no changes in the level of sucrose-dependent adhesion in spite of the addition of rGTFD. Further, the binding of rGbpC protein to the glucan synthesized by rGTFD was significantly higher than that to the glucan synthesized by either rGTFB or rGTFC. These results suggest that GbpC protein may play an important role in sucrose-dependent adhesion by binding to the soluble glucan synthesized by GTFD.