Abnormal PMC microtubule distribution pattern and chromosome behavior resulted in low pollen fertility of an intersubspecific autotetraploid rice hybrid
Abnormal PMC microtubule distribution pattern and chromosome behavior resulted in low pollen fertility of an intersubspecific autotetraploid rice hybrid
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异常的PMC微管分布模式和染色体行为导致亚种间同源四倍体水稻杂交种花粉育性低
DOI：
10.1007/s00606-010-0386-y
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发表时间：
2011-02-01
影响因子：
1.9
通讯作者：
Lu, Yong-Gen
中科院分区：
文献类型：
--
作者：
He, Jin-Hua;Shahid, Muhammad Qasim;Lu, Yong-Gen
同源四倍体水稻亚种间杂交种在营养生长和生殖生长方面都具有很高的杂种优势，但结实率低，阻碍了同源四倍体水稻的商业化利用。本研究以同源四倍体水稻高、低花粉育性杂交种为材料，采用间接免疫荧光激光扫描共聚焦显微镜技术，比较了花粉母细胞减数分裂过程中微管的分布和染色体行为。同源四倍体杂种的微管分布模式与二倍体水稻相似，但在花粉育性和结实率较低的杂种中发现了许多不同的异常。微管结构异常，包括粗线期微管结构畸变，终变期核周微管松散排列，中期I和中期II纺锤体异常，即多极、纺锤体极焦点丢失、纺锤体大小异常等。有些细胞发育成三联体，但没有形成四分体。染色体行为异常包括多价体比例高、染色体落后、染色体桥和微核。所有这些异常在低育性杂种中比在高育性杂种中更常见。这些结果表明，微管分布异常是影响同源四倍体水稻花粉育性和结实率的重要因素，并可能与染色体行为密切相关。
Intersubspecific autotetraploid rice hybrids have high heterosis in both vegetative and reproductive growth, but low seed set hinders commercial utilization of autotetraploid rice. Autotetraploid rice hybrids with high and low pollen fertility were used in the present study to compare microtubule distribution patterns and chromosome behavior during pollen mother cell (PMC) meiosis, using indirect immunofluorescence laser scanning confocal microscopy. Microtubule distribution patterns of autotetraploid hybrids were similar to diploid rice, but many different kinds of abnormalities were found in the hybrid with low pollen fertility and seed set. Abnormal microtubule organization including structurally distorted microtubules at pachytene, loosely knitted perinuclear microtubules at diakinesis during prophase I, and abnormal spindles, viz. multipoles, loss of spindle pole focus, abnormal size of spindles, and so on, were found at metaphase I and metaphase II. Some cells developed into triad with no formation of tetrad. Abnormal chromosome behaviors included high percentage of multivalents, chromosome lagging, chromosome bridges, and micronuclei. All these abnormalities were found more frequently in low-fertile hybrid than in high-fertile hybrid. These results suggest that abnormal microtubule distribution pattern is an important factor which affects pollen fertility and percentage seed set in autotetraploid rice, and may also have a close relationship with chromosome behavior.