Isozymes of myosin in growing and regenerating rat muscles.
Isozymes of myosin in growing and regenerating rat muscles.
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大鼠肌肉生长和再生中的肌球蛋白同工酶。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
E. Ghins
中科院分区：
文献类型：
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作者：
G. Maréchal;K. Schwartz;G. Beckers;E. Ghins
大鼠肌肉的天然肌球蛋白同工酶已在非解离条件下通过电泳分离出来。使用大肠杆菌肌球蛋白作为内标测量它们的迁移率，并通过电泳图的计算机平面测定法确定它们的相对浓度。在出生前三天的新生儿肌肉中观察到三种同工酶，在趾长伸肌（EDL）中观察到两种同工酶，在比目鱼肌（SOL）中观察到四种同工酶。成年动物切碎的 EDL 或 SOL 肌肉的再生在手术后第三天没有天然肌球蛋白；它们在手术后 15 天与新生儿肌肉相似，在手术后 60 天与成人肌肉相似。
Native myosin isozymes of rat muscles have been isolated by electrophoreses in non-dissociating conditions. Their mobilities were measured, using taenia coli myosin as an internal standard and their relative concentrations were determined by computer planimetry of the electrophoretograms. Three isozymes were observed in extensor digitorum longus (EDL), two in soleus (SOL), four in neonatal muscles three days before birth. Regenerates of minced EDL or SOL muscles in adult animals had no native myosin the third day after surgery; they were similar to neonatal muscles 15 days after surgery and to adult muscles 60 days after surgery.