Identification of naturally processed viral nonapeptides allows their quantification in infected cells and suggests an allele-specific T cell epitope forecast.
Identification of naturally processed viral nonapeptides allows their quantification in infected cells and suggests an allele-specific T cell epitope forecast.
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DOI：
10.1084/jem.174.2.425
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发表时间：
1991-08-01
期刊：
影响因子：
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通讯作者：
Rammensee HG
中科院分区：
文献类型：
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作者：
Falk K;Rötzschke O;Deres K;Metzger J;Jung G;Rammensee HG
病毒特异性细胞毒性T淋巴细胞(CTL)识别病毒感染细胞上主要组织相容性复合体(MHC)I类分子呈递的病毒源性多肽。这些多肽已经从感染细胞中分离出来，并与合成的多肽进行了比较。我们以前发现，KD或DB限制性的天然流感核蛋白多肽可以在反相高效液相色谱柱上流动，合成的多肽制剂中存在某些多肽副产物。在这里，我们通过广泛的生化和免疫学比较表明，天然多肽在所有方面都表现为推测的合成非肽，因此，必须与它们相同。通过与限定数量的纯合成非肽进行比较，可以确定感染细胞中含有的这些天然多肽的绝对量在220到540个拷贝之间。将天然的KD限制性多肽与已发表的含有其他KD限制性CTL表位的合成肽进行比较，提出了一种新的MHC等位基因特异性T细胞表位预测方法，该方法基于9个氨基酸残基的定义长度和第二位和最后一位关键氨基酸残基。
Virus-specific cytotoxic T lymphocytes (CTL) recognize virus-derived peptides presented by major histocompatibility complex (MHC) class I molecules on virus-infected cells. Such peptides have been isolated from infected cells and were compared to synthetic peptides. We found previously the Kd- or Db-restricted natural influenza nucleoprotein peptides to coelute on reversed phase high performance liquid chromatography columns with certain peptidic by-products present in synthetic peptide preparations. Here we show by extensive biochemical and immunological comparison that the natural peptides in all respects behave as the surmised synthetic nonapeptides, and thus, must be identical to them. The absolute amounts of these natural peptides contained in infected cells could be determined to be between 220 and 540 copies by comparing with defined amounts of pure synthetic nonapeptides. The comparison of the natural Kd-restricted peptide with published synthetic peptides known to contain other Kd-restricted CTL epitopes suggested a new MHC allele-specific T cell epitope forecast method, based on the defined length of nine amino acid residues and on critical amino acid residues at the second and the last position.