Fish stress become visible: A new attempt to use biosensor for real-time monitoring fish stress
Fish stress become visible: A new attempt to use biosensor for real-time monitoring fish stress
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鱼类应激变得可见：利用生物传感器实时监测鱼类应激的新尝试
DOI：
10.1016/j.bios.2014.09.015
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发表时间：
2015
影响因子：
12.6
通讯作者：
H. Endo
中科院分区：
文献类型：
--
作者：
H. Wu;A. Aoki;T. Arimoto;T. Nakano;H. Ohnuki;M. Murata;H. Ren;H. Endo
为了避免鱼类死亡并提高生产率，必须监测养殖鱼类的生理条件，包括应激状态。作为压力的重要指标，葡萄糖浓度是通过体外血液分析来监测的。鱼类在环境条件下的生理过程在很多方面都比陆生动物经历的更为严酷。此外，分析前麻醉和捕获鱼的过程可能会产生不准确的结果。为了解决这些问题，我们开发了无线生物传感器系统来监测鱼类的生理状况。该系统可实现人工无应激和非致死分析，并可对鱼类应激进行可靠的实时监测。该生物传感器包括作为工作电极的 Pt-Ir 线和作为参比电极的 Ag/AgCl 浆料。使用戊二醛将葡萄糖氧化酶固定在工作电极上。我们使用眼球间质巩膜液（EISF）作为传感器的体内植入部位，其成分浓度与血液成分浓度有很好的相关性。在本研究中，我们研究了由于水化学变化引起的压力，包括溶解氧、pH 值和氨氮化合物。还监测了从行为互动（包括攻击行为和视觉刺激）中感受到的压力。水化学变化导致葡萄糖浓度（压力）增加，随着刺激的消除而减少。对于行为互动，压力水平随着回避、感官行为和活动而变化。我们相信，所提出的生物传感器系统可用于快速、可靠、方便地分析鱼类的生理状况，并准确反映鱼类所经历的应激。
To avoid fish mortality and improve productivity, the physiological conditions including stress state of the cultured fish must be monitored. As an important indicator of stress, glucose concentrations are monitored usingin vitroblood analysis. The physiological processes of fish under environmental conditions are harsher in many ways than those experienced by terrestrial animals. Moreover, the process of anaesthetizing and capturing the fish prior to analysis may produce inaccurate results. To solve these problems, we developed wireless biosensor system to monitor the physiological condition of fish. This system enables artificial stress-free and non-lethal analysis, and allows for reliable real-time monitoring of fish stress. The biosensor comprised Pt–Ir wire as the working electrode and Ag/AgCl paste as the reference electrode. Glucose oxidase was immobilized on the working electrode using glutaraldehyde. We used the eyeball interstitial sclera fluid (EISF) as thein vivoimplantation site of the sensor, which component concentration correlates well with that of blood component concentration. In the present study, we investigated stress due to alterations in water chemistry, including dissolved oxygen, pH, and ammonia–nitrogen compounds. Stress perceived from behavioural interactions, including attacking behaviour and visual irritation, was also monitored. Water chemistry alterations induced increases in the glucose concentration (stress) that decreased with removal of the stimulus. For behavioural interactions, stress levels change with avoidance, sensory behaviour and activity. We believe that the proposed biosensor system could be useful for rapid, reliable, and convenient analysis of the fish physiological condition and accurately reflects the stress experienced by fish.