[H-3]SCH-23390 BINDING TO D1 DOPAMINE-RECEPTORS IN THE BASAL GANGLIA OF THE CAT AND PRIMATE - DELINEATION OF STRIOSOMAL COMPARTMENTS AND PALLIDAL AND NIGRAL SUBDIVISIONS
[H-3]SCH-23390 BINDING TO D1 DOPAMINE-RECEPTORS IN THE BASAL GANGLIA OF THE CAT AND PRIMATE - DELINEATION OF STRIOSOMAL COMPARTMENTS AND PALLIDAL AND NIGRAL SUBDIVISIONS
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DOI：
10.1016/0306-4522(88)90130-3
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发表时间：
1988-07-01
期刊：
影响因子：
3.3
通讯作者：
NASTUK, MA
中科院分区：
文献类型：
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作者：
BESSON, MJ;GRAYBIEL, AM;NASTUK, MA
对猫、猴和人基底节区D1多巴胺受体的分布进行了放射自显影研究。这些受体结合位点直接用d1选择性拮抗剂[3H]SCH 23390标记，并同时进行配体结合试验。序列或同切片分析允许比较D1结合分布、乙酰胆碱酯酶染色和酪氨酸羟化酶免疫反应性。在所有被研究的物种中，背纹状体与缺乏乙酰胆碱酯酶的纹状体呈斑块状或特别密集的D1结合。腹侧纹状体中存在高度图案化的结合。在白球和黑质的分支之间观察到结合密度的差异。钯的外节段D1结合极为稀疏，而内节段（或猫的髓内核）是D1结合密度高的部位。结合密度在内段核心处最大，酪氨酸羟化酶阳性纤维通过该核心包围并微弱分散。腹侧苍白球存在弱结合。在黑质中，网状部表现出最密集的结合，特别是内侧。致密部的结合更稀疏，但部分酪氨酸羟酶阳性神经元树突向腹侧延伸至网状部的D1密集结合区。我们得出结论，[3H]SCH 23390定义的D1结合在背纹状体中被区隔化，特别是与报道的纹状体D2多巴胺受体的分布有关，这可能在纹状体内神经传递以及传入和传出神经传递的多巴胺能调节中具有功能意义。D1受体在黑质中的分离定位表明网状部的主要激活，包括靠近致密细胞带的腹侧和内侧区域。从纹状体的室室到白质的细分的特定途径也可能通过不同的受体亚型被多巴胺不同地调节。在苍白球的水平上，这种D1调节似乎仅限于投射到丘脑的内部部分，而不是投射到丘脑底核的外部苍白球。
The distribution of D1 dopamine receptors was studied autoradiographically in the basal ganglia of the cat, monkey and human. These receptor binding sites were labeled directly with the D1-selective antagonist [3H]SCH 23390, and ligand-binding assays were performed concurrently. Serial- or same-section analysis permitted comparisons among D1 binding distributions, acetylcholinesterase staining and tyrosine hydroxylase immunoreactivity. In all species studied, the dorsal striatum exhibited patches or particularly dense D1 binding in correspondence with acetylcholinesterase-poor striosomes. Highly patterned binding was present in the ventral striatum. Distinctions in binding density were observed among the subdivisions of the globus pallidus and of the substantia nigra. The external segment of the palladium was extremely sparse in D1 binding, whereas the internal segment (or entopeduncular nucleus in the cat) was a site of high D1 binding density. The binding density was greatest in the core of the internal segment, and tyrosine hydroxylase-positive fibers surrounded and weakly dispersed themselves through this core. Weak binding was present in the ventral pallidum. In the substantia nigra, the pars reticulata demonstrated the densest binding, particularly medially. The pars compacta showed much sparser binding, though some of its tyrosine hydroxylase-positive neurons had dendrites extending ventrally into the zone of dense D1 binding in the pars reticulata. We conclude that [3H]SCH 23390-defined D1 binding is compartmentalized in the dorsal striatum and that, particularly in relation to the reported distributions of striatal D2 dopamine receptors, this is likely to be of functional significance in the dopaminergic modulation of intrastriatal neurotransmission as well as of afferent and efferent neurotransmission. The segregated localizations of D1 receptors in the substantia nigra suggst predominant activation of the pars reticulata, including ventral and medial regions adjacent to the densocellular zone. Specific pathways from compartments in the striatum to subdivision of the pallidum may also be differentially modulated by dopamine acting via distinct receptor subtypes. At the level of the pallidum, such D1 modulation appears to be restricted to the internal segment, which projects to the thalamus, rather than to the external pallidum, which projects to the subthalamic nucleus.