Effect of volume of blood cultured on detection of Streptococcus viridans bacteraemia.
Effect of volume of blood cultured on detection of Streptococcus viridans bacteraemia.
复制标题
血培养量对草绿色链球菌菌血症检测的影响。
DOI：
10.1136/jcp.37.5.568
复制
发表时间：
1984
影响因子：
3.4
通讯作者：
D. Wilson
中科院分区：
文献类型：
--
作者：
D. Shanson;F. Thomas;D. Wilson
58名拔牙患者每人采集45 ml血液。将其分为30 ml和15 ml血样进行培养。将30 ml样品接种到120 ml含0.05%类液的营养肉汤中，将15 ml样品接种到60 ml相同肉汤中，使肉汤中血液的最终稀释度始终为15。30 ml和15 ml血培养分别检出草绿色链球菌血症27例和15例。只有一例链球菌菌血症是通过对采集的总体积血液（45 ml）进行培养而不是仅对30 ml血液样品进行培养而检测到的。这些发现表明，30 ml血液的培养物导致比仅15 ml血液的培养物多检测到高达80%的产生草绿色链球菌的血液培养物。每次培养收集超过30 ml血液不太可能证明是值得的。因此，在对疑似感染性心内膜炎患者进行调查时，每次草绿色链球菌培养的最佳采血量可能为30 ml，而不是15 ml。
Fifty eight patients undergoing dental extraction each had 45 ml blood collected. This was divided into 30 ml and 15 ml blood samples for culture. The 30 ml sample was inoculated into 120 ml nutrient broth with 0.05% liquoid and the 15 ml sample into 60 ml of identical broth so that the final dilution of blood in broth was always 1/5. Bacteraemia due to viridans streptococci was found in 27 and 15 patients by culturing the 30 ml and 15 ml blood samples respectively. Only one further case of streptococcal bacteraemia was detected by culture of the total volume of blood collected (45 ml) rather than culture of the 30 ml blood sample alone. These findings suggest that the culture of 30 ml blood results in the detection of up to 80% more blood cultures yielding Streptococcus viridans than the culture of only 15 ml blood. The collection of more than 30 ml blood for each culture is unlikely to prove worthwhile. It is suggested that 30 ml rather than 15 ml blood is probably the optimal volume of blood for each culture of S viridans when patients with suspected infective endocarditis are investigated.