ALCOHOL INHIBITS CELL MITOSIS IN G(2)-M PHASE IN CELL-CYCLE IN A HUMAN-LYMPHOCYTES IN-VITRO STUDY
ALCOHOL INHIBITS CELL MITOSIS IN G(2)-M PHASE IN CELL-CYCLE IN A HUMAN-LYMPHOCYTES IN-VITRO STUDY
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DOI：
10.1016/0741-8329(95)02008-x
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发表时间：
1995-11-01
期刊：
影响因子：
2.3
通讯作者：
RAJGURU, SU
中科院分区：
文献类型：
--
作者：
AHLUWALIA, BS;WESTNEY, LS;RAJGURU, SU
酗酒与免疫能力丧失有关，这会导致对感染的抵抗力下降。没有单一的机制可以解释酒精对身体防御机制的有害影响。我们提供的数据表明，在培养的淋巴细胞中，培养基中10 - 40毫摩尔的酒精会使细胞有丝分裂减少18 - 90%（p < 0.001）。在酒精浓度达到40毫摩尔时，细胞有丝分裂呈线性下降；在100毫摩尔时，细胞有丝分裂几乎停止。本研究旨在确定酒精在细胞周期的哪个阶段产生影响。结果显示，在酒精浓度达到50毫摩尔时，DNA合成未受影响，这表明细胞周期中的G₁ - S期未受影响。在100毫摩尔酒精浓度下，DNA合成显著下降（p < 0.01）。根据这项研究的结果，我们得出结论：亚药理学剂量水平的酒精（10毫摩尔）显著抑制细胞有丝分裂，并且酒精的抑制作用是在细胞周期的G₂ - M期产生的，G₁ - S期未受影响。
Alcohol abuse is associated with the loss of immunocompetence, which leads to decreasing resistance to infections. No single mechanism can be accountable for the detrimental effects of alcohol on the body's defense mechanism. We present data demonstrating that, in cultured lymphocytes, 10-40 mM alcohol in the media caused 18-90% decrease in cell mitosis (p < 0.001). There was a linear decrease in cell mitosis upto 40 mM alcohol; at 100 mM cell mitosis virtually ceased. This study aimed to determine in which phase of the cell cycle did alcohol mediate its effects. The results showed that DNA synthesis was not affected with up to 50 mM alcohol, suggesting that G(1)-S phase in the cell cycle remained unaffected. At 100 mM alcohol, DNA synthesis decreased significantly (p < 0.01). From the results of this study, we conclude that a subpharmacological dose level of alcohol (10 mM) significantly inhibited cell mitosis and the inhibitory effect of alcohol was mediated in the G(2)-M phase in the cell cycle. The G(1)-S phase was unaffected.