Direct transcriptional activation of BT genes by NLP transcription factors is a key component of the nitrate response in Arabidopsis
Direct transcriptional activation of BT genes by NLP transcription factors is a key component of the nitrate response in Arabidopsis
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DOI：
10.1016/j.bbrc.2016.12.135
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发表时间：
2017-01-29
影响因子：
3.1
通讯作者：
Yanagisawa, Shuichi
中科院分区：
文献类型：
--
作者：
Sato, Takeo;Maekawa, Shugo;Yanagisawa, Shuichi
硝酸盐调节生长和发育，在植物中起营养信号的作用。尽管许多生理过程中对硝酸盐的反应被很好地描述为硝酸盐反应，但硝酸盐反应的分子机制还没有完全被理解。在这里，我们证明了NLP转录因子作为硝酸盐反应的关键调节因子，直接激活了NLP诱导的BT1和BT2的表达，这些蛋白编码了拟南芥中具有植物特有结构域结构的假定支架蛋白。有趣的是，35S启动子驱动的BT2表达在一定程度上挽救了因NLP活性降低而导致的拟南芥生长抑制。此外，BT1和BT2的同时中断影响了硝酸盐依赖的侧根的发育。这些结果表明，NLP转录激活子直接激活BT1和BT2是拟南芥硝酸盐反应分子机制的关键组成部分。(C)2016 Elsevier Inc.保留所有权利。
Nitrate modulates growth and development, functioning as a nutrient signal in plants. Although many changes in physiological processes in response to nitrate have been well characterized as nitrate responses, the molecular mechanisms underlying the nitrate response are not yet fully understood. Here, we show that NLP transcription factors, which are key regulators of the nitrate response, directly activate the nitrate-inducible expression of BT1 and BT2 encoding putative scaffold proteins with a plant-specific domain structure in Arabidopsis. Interestingly, the 35S promoter-driven expression of BT2 partially rescued growth inhibition caused by reductions in NLP activity in Arabidopsis. Furthermore, simultaneous disruption of BT1 and BT2 affected nitrate-dependent lateral root development. These results suggest that direct activation of BT1 and BT2 by NLP transcriptional activators is a key component of the molecular mechanism underlying the nitrate response in Arabidopsis. (C) 2016 Elsevier Inc. All rights reserved.