Neural circuitry of a polycystin-mediated hydrodynamic startle response for predator avoidance
Neural circuitry of a polycystin-mediated hydrodynamic startle response for predator avoidance
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DOI：
10.7554/elife.36262
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发表时间：
2018-12-14
期刊：
影响因子：
7.7
通讯作者：
Jekely, Gaspar
中科院分区：
文献类型：
--
作者：
Bezares-Calderon, Luis A.;Berger, Juergen;Jekely, Gaspar
由厌恶刺激（包括捕食者）引发的惊吓反应在动物中普遍存在。这些协调的全身动作需要快速、同时激活大量肌肉。在这里，我们研究浮游幼虫的惊吓反应，以了解该行为的全身回路实现。当遇到水的振动时，环节动物扁沙蚕的幼虫会关闭其运动纤毛，同时抬起副足。该反应由表达多囊蛋白 PKD1-1 和 PKD2-1 的项圈受体神经元介导。 CRISPR 生成的 PKD1-1 和 PKD2-1 突变幼虫不会受到惊吓，并且会以更高的速度成为桡足类捕食者的猎物。颈圈受体细胞电路的全身连接体的重建揭示了睫状带和肌肉的前馈电路的汇聚。接线图提出了响应的节间和左右协调的电路机制。我们的结果揭示了多囊蛋白介导的机械感觉如何触发参与躲避捕食者的协调全身效应器反应。
Startle responses triggered by aversive stimuli including predators are widespread across animals. These coordinated whole-body actions require the rapid and simultaneous activation of a large number of muscles. Here we study a startle response in a planktonic larva to understand the whole-body circuit implementation of the behaviour. Upon encountering water vibrations, larvae of the annelid Platynereis close their locomotor cilia and simultaneously raise the parapodia. The response is mediated by collar receptor neurons expressing the polycystins PKD1-1 and PKD2-1. CRISPR-generated PKD1-1 and PKD2-1 mutant larvae do not startle and fall prey to a copepod predator at a higher rate. Reconstruction of the whole-body connectome of the collar-receptor-cell circuitry revealed converging feedforward circuits to the ciliary bands and muscles. The wiring diagram suggests circuit mechanisms for the intersegmental and left-right coordination of the response. Our results reveal how polycystin-mediated mechanosensation can trigger a coordinated whole-body effector response involved in predator avoidance.