Defining the structure and function of acyl-homoserine lactone autoinducers.
Defining the structure and function of acyl-homoserine lactone autoinducers.
复制标题
定义酰基高丝氨酸内酯自诱导剂的结构和功能。
DOI：
10.1007/978-1-60761-971-0_12
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Uhlson,CharisL
中科院分区：
文献类型：
--
作者：
Churchill,MairEA;Sibhatu,HiruyM;Uhlson,CharisL
群体感应在调节细菌的共生和致病关系中起着重要作用，近年来引起了人们的广泛关注。酰基高丝氨酸内酯（AHL）是在与真核生物相互作用的许多革兰氏阴性细菌中发现的群体感应基因调节过程中的重要信号分子。AHLs由酰基高丝氨酸内酯脱氢酶产生。细菌可以有多个阿勒合酶基因，这些物种可能会产生几种不同类型的AHL。确定阿勒合成酶在不同条件下产生的AHLs的类型和相对量为了解阿勒合成酶的功能机制和群体感应信号启动的转录级联调节提供了重要的见解。本章描述了用于确定样品中存在的AHLs类型和相对量的质谱法。
Quorum sensing plays a central role in regulating many community-derived symbiotic and pathogenic relationships of bacteria, and as such has attracted much attention in recent years. Acyl-homoserine lactones (AHLs) are important signaling molecules in the quorum sensing gene-regulatory processes found in numerous gram-negative species of bacteria that interact with eukaryotic organisms. AHLs are produced by acyl-homoserine lactone synthases. Bacteria can have multiple genes for AHL synthase enzymes, and such species are likely to produce several different types of AHLs. Determination of the types and the relative amounts of AHLs produced by AHL synthases in bacteria under varied conditions provides important insights into the mechanism of AHL synthase function and the regulation of transcriptional cascades initiated by quorum sensing signaling. This chapter describes a mass spectrometry method for determining the types and relative amounts of AHLs present in a sample.