Microscale two-dimensional (2D) temperature mapping by ratiometric fluorescence imaging under orthogonal excitations
Microscale two-dimensional (2D) temperature mapping by ratiometric fluorescence imaging under orthogonal excitations
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DOI：
10.1016/j.expthermflusci.2018.02.009
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发表时间：
2018-06-01
影响因子：
3.2
通讯作者：
Pan, Liang
中科院分区：
文献类型：
--
作者：
Chen, Chen;Shen, Tong;Pan, Liang
液体中的微尺度温度映射在微流体学和生物学等许多研究领域都具有重要意义。在目前的测温方法中，使用荧光的光学探测是特别可取的，因为它的高空间分辨率和非侵入性。在这里，我们报告了一种新的微尺度二维（2D）荧光测温。该方法利用旋转分子运动的温度依赖性及其对荧光的去偏振的影响，通过测量正交偏振激发的荧光强度的差异。用一个电荷耦合器件（CCD）相机，我们得到了二维比例映射的温度从交替偏振激发下连续记录的荧光图像。我们证明了可靠的温度映射在液体中，在低于1摄氏度的温度精度和低于10 μ m的空间分辨率。我们还表明，所提出的测温方法是强大的荧光强度变化，适合2D映射和快速读出，是可比的CCD帧速率。此外，它很容易被集成到显微镜系统，因为只有激发偏振旋转是必需的。
Microscale temperature mapping in liquids is of great importance in many areas of research such as microfluidics and biology. Among the current thermometric approaches, optical probing using fluorescence is particularly desirable because of its high spatial resolution and non-invasive nature. Here we report a new microscale two-dimensional (2D) fluorescence thermometry. This method exploits the temperature dependence of rotational molecular motion and its influence on the depolarization of fluorescence light, by measuring the difference in fluorescence intensities excited by orthogonal polarizations. With one charge coupled device (CCD) camera, we get 2D ratiometric mappings of temperature from successively recorded fluorescence images under alternatively polarized excitations. We demonstrate reliable temperature mapping in liquids at sub-1 degrees C temperature accuracy and sub-10 mu m spatial resolution. We also show that the proposed thermometry approach is robust against fluorescence intensity variations, suitable for 2D mapping and of fast readout that is comparable to CCD framerate. Moreover, it is easy to be integrated into microscope systems since only rotation of excitation polarization is needed.