Pax2a is expressed in oocytes and is responsible for early development and oogenesis in zebrafish
Pax2a is expressed in oocytes and is responsible for early development and oogenesis in zebrafish
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Pax2a 在卵母细胞中表达，负责斑马鱼的早期发育和卵子发生
DOI：
10.1016/j.bbrc.2020.09.059
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发表时间：
2020
影响因子：
3.1
通讯作者：
Md. Hasan Ali and Toshinobu Tokumoto
中科院分区：
文献类型：
--
作者：
Theeranukul Pachoensuk;Taketo Fukuyo;Klangnurak Wanlada;Md. Rezanujjaman;Md. Mostafizur Rahaman;Kagura Sasaoka;Md. Maisum Sarwar Jyoti;Md. Rubel Rana;Md. Hasan Ali and Toshinobu Tokumoto
我们在前期的研究中，通过基因芯片分析和RNA测序，筛选出pax 2a等11个基因作为排卵诱导基因的候选基因。本研究的目的是探讨thepax 2agene在排卵诱导过程中的作用。本研究建立了一个缺失6个核苷酸的F2 pax 2a纯合突变体斑马鱼。然而，该缺失包括pax 2agene的起始密码子（ATG），并且仍然检测到Pax 2a蛋白，这表明该缺失导致起始密码子移动到下一个ATG，导致12个氨基酸的缺失。F2 pax 2a纯合突变斑马鱼显示排卵。然而，胚胎在外包阶段表现出异常的椭圆形，导致蛋黄和尾巴形成异常和心脏水肿。存活的F3纯合突变体不发育卵巢。Pax 2a在卵母细胞和卵子中被检测到，但在Prim-22期后未被检测到。提示pax 2基因在卵母细胞中作为一个母系基因表达，是卵子发生和早期发育所必需的。
Eleven genes, includingpax2a,were selected as candidate ovulation-inducing genes on the basis of microarray analysis and RNA sequencing in our previous study. The purpose of this study was to investigate the role of thepax2agene in the ovulation-inducing process. F2pax2ahomozygous mutant zebrafish possessing a deletion of 6 nucleotides were established in this study. However, the deletion included the start codon (ATG) of thepax2agene, and the Pax2a protein was still detected, which indicated that the deletion caused a shift in the start codon to the next ATG, resulting in a 12-amino acid deletion. F2pax2ahomozygous mutant zebrafish showed ovulation. However, the embryos showed an abnormal oval shape at the epiboly stage that resulted in yolk and tail formation abnormalities and heart edema. The surviving F3 homozygous mutants did not develop ovaries. Pax2a was detected in oocytes and eggs but not after the Prim-22 stage. It is suggested thatpax2ais expressed as a maternal gene in oocytes and is necessary for oogenesis and early development.