Seminal plug expulsion induced by electrical stimulation of the intermesenteric nerve in anesthetized rats
Seminal plug expulsion induced by electrical stimulation of the intermesenteric nerve in anesthetized rats
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DOI：
10.1095/biolreprod.107.060921
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发表时间：
2007-10-01
影响因子：
3.6
通讯作者：
Giuliano, Francois
中科院分区：
文献类型：
--
作者：
Bernabe, Jacques;Clement, Pierre;Giuliano, Francois
顺行射精需要同时激活分别通向精道（包括膀胱颈和骨盆会阴横纹肌）的自主神经和躯体神经。本研究旨在探讨肠系膜间神经（IMNs）在麻醉大鼠射精过程中的作用。在异氟烷麻醉的雄性大鼠中测试了完整IMN和切片IMN的远端和近端残端的电刺激。完整的IMN的电刺激也适用于大鼠急性脊髓横断在T8水平。通过测量精囊压力（SVP）和球海绵体肌（BS）的收缩，分别评价IMN电刺激对射精的发射和排出阶段的影响。电刺激IMN可引起SVP增加和BS肌节律性收缩，同时伴有精栓排出。与完整的IMN电刺激相比，射精和有节奏的BS肌肉收缩的发生，但不是SVP增加，减少了电刺激的远端残端的切片IMN。与完整的IMN电刺激相比，射精和节律性BS肌肉收缩的发生没有显着修改，而SVP的增加减少时，切片IMN的近端残端被刺激。Spinalization取消射精和节奏BS肌肉收缩，但不损害SVP增加。结果表明，IMN的传入和脊髓上的中继以及IMN的传出均参与了刺激IMN诱发射精的过程。我们建议，IMN电刺激范例可用于研究射精的生理和药理学方面。
Synchronized activation of autonomic and somatic divisions of the nervous system respectively destined to the seminal tract, including the bladder neck and the pelvi-perineal striated musculature, is necessary for anterograde ejaculation. We aimed at investigating the role of intermesenteric nerves (IMNs) in ejaculation in anesthetized rats. Electrical stimulation of intact IMNs and distal and proximal stumps of the sectioned IMN were tested in isoflurane-anesthetized male rats. Electrical stimulation of the intact IMN was also applied to rats with acute spinal transection at the T8 level. The effects of IMN electrical stimulation on emission and expulsion phases of ejaculation were evaluated by measuring seminal vesicle pressure (SVP) and bulbospongiosus (BS) muscle contractions, respectively. IMN electrical stimulation could induce SVP increase and rhythmic contractions of BS muscle concomitantly with expulsion of the seminal plug. When compared with intact IMN electrical stimulation, the occurrence of ejaculation and rhythmic BS muscle contractions, but not SVP increase, was reduced in response to electrical stimulation of the distal stump of the sectioned IMN. In comparison to intact IMN electrical stimulation, the occurrence of ejaculation and rhythmic BS muscle contractions was not significantly modified, whereas the increase in SVP was diminished when the proximal stump of the sectioned IMN was stimulated. Spinalization abolished ejaculation and rhythmic BS muscle contraction but did not impair SVP increase. It is concluded that both afferents conveyed by IMN and relaying supraspinally and efferents of IMN are involved in IMN electrical stimulation-induced ejaculation. We propose that the IMN electrical stimulation paradigm can be used to investigate physiological and pharmacologic aspects of ejaculation.