ROLE OF YADA IN RESISTANCE OF YERSINIA-ENTEROCOLITICA TO PHAGOCYTOSIS BY HUMAN POLYMORPHONUCLEAR LEUKOCYTES
ROLE OF YADA IN RESISTANCE OF YERSINIA-ENTEROCOLITICA TO PHAGOCYTOSIS BY HUMAN POLYMORPHONUCLEAR LEUKOCYTES
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DOI：
10.1128/iai.62.4.1275-1281.1994
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发表时间：
1994-04-01
影响因子：
3.1
通讯作者：
CORNELIS, GR
中科院分区：
文献类型：
--
作者：
CHINA, B;NGUYEN, BT;CORNELIS, GR
致病性小肠结肠炎耶尔森菌细胞不诱导人多形核白细胞（PMNs）的化学发光反应。我们测试了对Y的化学发光响应。已知pYV编码因子中受影响的小肠结肠炎突变体。我们没有发现任何影响的Yops在这一现象。相比之下，YadA的存在与化学发光的缺乏相关。YadA在细菌表面的表达也降低了PMN的吞噬作用。最后，我们测量了Y的存活率。小肠结肠炎细胞面对中性粒细胞的经典平板法和一种新的光度测定法。我们观察到YadA（+）细菌没有被杀死，而YadA（-）细菌被杀死。我们的结论是，YadA在Y.小肠结肠炎细胞阻止中性粒细胞的吞噬和杀伤。这一结论与我们最近观察到的YadA保护Y.小肠结肠炎菌通过C3 b的调理作用。
Pathogenic Yersinia enterocolitica cells do not induce the chemiluminescence response of human polymor-phonuclear leukocytes (PMNs). We tested the chemiluminescence response to Y. enterocolitica mutants affected in the known pYV-encoded factors. We did not detect any influence of the Yops in this phenomenon. By contrast, the presence of YadA correlated with a lack of chemiluminescence. The expression of YadA at the bacterial surface also reduced the phagocytosis by PMNs. Finally, we measured the survival of Y. enterocolitica cells confronted with PMNs by the classical plating method and by a new luminometry assay. We observed that YadA(+) bacteria were not killed, while YadA(-) bacteria were killed. We conclude that the presence of YadA at the surface of Y. enterocolitica cells prevents phagocytosis and killing by PMNs. This conclusion is in good agreement with our recent observation that YadA protects Y. enterocolitica from opsonization by C3b.